CD36 deficiency ameliorates drug-induced acute liver injury in mice.

Molecular Medicine
Chen ZhangQiang You

Abstract

Acetaminophen (APAP) overdose causes hepatotoxicity and even acute liver failure. Recent studies indicate that sterile inflammation and innate immune cells may play important roles in damage-induced hepatocytes regeneration and liver repair. The scavenger receptor CD36 has its crucial functions in sterile inflammation. However, the roles of CD36 in APAP induced acute liver injury remain unclear and warrant further investigation. WT C57BL/6 J and CD36-/- mice were intraperitoneally injected with APAP (300 mg/kg) after fasting for 16 h. Liver injury was evaluated by serum alanine aminotransferase (ALT) level and liver tissue hematoxylin and eosin (H&E) staining. Liver inflammatory factor expression was determined by real-time polymerase chain reaction (PCR). The protein adducts forming from the metabolite of APAP and the metabolism enzyme cytochrome P450 2E1 (CYP2E1) levels were measured by Western blot. Liver infiltrating macrophages and neutrophils were characterized by flow cytometry. RNA sequencing and Western blot were used to evaluate the effect of damage-associated molecular patterns (DAMP) molecule high mobility group B1 (HMGB1) on WT and CD36-/- macrophages. Moreover, PP2, a Src kinase inhibitor, blocking CD36 signaling,...Continue Reading

References

Jun 1, 1989·Archives of Biochemistry and Biophysics·J L RaucyM Black
May 17, 1996·The Journal of Biological Chemistry·S S LeeF J Gonzalez
Oct 17, 1998·Toxicology and Applied Pharmacology·H ZaherF J Gonzalez
Sep 19, 2003·Biochemical and Biophysical Research Communications·Laura P JamesJack A Hinson
May 16, 2006·Gastroenterology·Robert F SchwabeDavid A Brenner
Sep 5, 2006·Cell Metabolism·S Ohidar RahamanRoy L Silverstein
Jun 17, 2008·Current Opinion in Immunology·Pascale JeanninYves Delneste
Aug 21, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Qiang YouCynthia Ju
Sep 16, 2008·The Journal of Nutritional Biochemistry·Jose-Manuel Fernández-RealWifredo Ricart
May 28, 2009·Science Signaling·Roy L Silverstein, Maria Febbraio
Sep 29, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Daniel J AntoineB Kevin Park
Dec 19, 2009·Handbook of Experimental Pharmacology·Jack A HinsonLaura P James
Jul 20, 2010·Toxicology and Applied Pharmacology·C David WilliamsHartmut Jaeschke
Nov 23, 2010·Nature Reviews. Immunology·Grace Y Chen, Gabriel Nuñez
Jul 13, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Hartmut JaeschkeMary L Bajt
Nov 3, 2011·The Journal of Pharmacology and Experimental Therapeutics·Xi YangWilliam F Salminen
Apr 26, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Pedro E MarquesGustavo Batista Menezes
Mar 7, 2013·Pharmaceutical Research·Mitchell R McGill, Hartmut Jaeschke
Jul 24, 2013·Biochemical Pharmacology·Qiang YouCynthia Ju
Jul 18, 2014·Journal of Leukocyte Biology·Meng WangCynthia Ju
Oct 22, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Peter LundbäckHelena Erlandsson Harris
Dec 4, 2016·Journal of Hepatology·Benjamin L Woolbright, Hartmut Jaeschke
Mar 16, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yong HeBin Gao
Dec 9, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Che XuQiang You
Aug 15, 2019·Theranostics·Jingchun Wang, Yongsheng Li
Feb 11, 2020·JHEP Reports : Innovation in Hepatology·Paul F LebeauRichard C Austin
Sep 23, 2020·Nature Communications·Jian-Wei HaoTong-Jin Zhao

❮ Previous
Next ❯

Software Mentioned

GraphPad Prism
FlowJo
UpSetR
Image J
VENN

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
Ken-ichi Hirano
Clinica Chimica Acta; International Journal of Clinical Chemistry
Makoto ImaiYasushi Kitaura
© 2021 Meta ULC. All rights reserved