CD4+ CD45RA+ T cells from adults respond to recall antigens after CD28 ligation

International Immunology
D PillingM Salmon

Abstract

The leukocyte common antigen isoforms CD45RA and CD45RO have long been used to discriminate human naive and memory T cells respectively. This model was largely based on the observation that CD45RO+ T cells respond preferentially to and show a higher frequency of precursors specific for recall antigens. However, CD45RA+ T cells have more stringent requirements for stimulation and standard in vitro assays may favour CD45RO+ cells in this respect. We tested the hypothesis that CD45RA+ T cells respond poorly to in vitro stimulation with recall antigens because of inadequate stimulation rather than a lack of precursors. Limiting dilution analyses (LDA) for tetanus toxoid (TT)-specific T cells were performed in the presence or absence of exogenous anti-CD28 antibody. Addition of anti-CD28 yielded no proliferation in the absence of specific antigen. The precursor frequency for TT in the CD4+ CD45RO+ population was approximately 1:4000, while the frequency of CD4+ CD45RA+ T cells specific for TT was 4- to > 20-fold lower. Addition of anti-CD28 antibody did not significantly alter the apparent precursor frequency for CD45RO+ cells but yielded an enhancement of the value for CD45RA+ cells by 3- to > 5-fold. No enhancement of antigen-spec...Continue Reading

Citations

Jun 1, 2001·European Journal of Immunology·E B BellS M Sparshott
Jan 31, 2002·Arthritis and Rheumatism·Marie L DaviesSimon J Bowman
Feb 10, 2000·Springer Seminars in Immunopathology·L R Wedderburn, P Woo
Feb 26, 2000·Vaccine·D F ToughJ Sprent
Aug 12, 1999·Clinical and Experimental Immunology·T RustemeyerR J Scheper
Jun 6, 2002·Immunology·Marion Berard, David F Tough
Nov 24, 1999·Immunological Reviews·D F ToughJ Sprent
Mar 22, 2002·Journal of Virology·Martina SesterHans Köhler
Mar 12, 2008·Journal of Leukocyte Biology·Diane D ShaoDarrell Pilling
Feb 10, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Satoshi NemotoJames J Mulé
Oct 13, 2009·Journal of Immunological Methods·Darrell PillingRichard H Gomer
Oct 31, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·A M Dyrhol-RiiseB Asjö
Dec 10, 1999·Rheumatology·M Salmon, C Gordon
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J M FaintM Salmon
Nov 25, 2003·Clinical and Experimental Immunology·D G HaegertV Gadag
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Darrell PillingRichard H Gomer
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mathis HeydtmannDavid H Adams
Dec 25, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joseph IllingworthFrancis M Ndungu
Jul 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ana SuárezCarmen Gutiérrez
Jul 27, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diletta Di MitriArne N Akbar
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael P WeekesAndrew J Carmichael
Mar 26, 1999·European Journal of Immunology·D PillingM Salmon

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