PMID: 9438207Jan 23, 1998Paper

CD4-mediated anchoring of the seminal antigen gp17 onto the spermatozoon surface

Human Immunology
P BergamoP Abrescia

Abstract

A soluble 1, kDa glycoprotein, namely gp17, was previously isolated from human semen and used to obtain mouse monoclonal or chicken polyclonal antibodies. This protein was shown to bind CD4+ T-cells and to soluble recombinant CD4 in vitro. Here, we report that the anti-gp17 monoclonal antibodies are captured by ejaculated spermatozoa and that gp17-like antigens are released by cell acid extraction. Immunoblotting experiments with monoclonal antibodies indicated that SDS-lysates from spermatozoa contain proteins with the same electrophoretic and antigenic properties of CD4 and gp17. Anti-CD4 mouse monoclonal antibodies were used to coprecipitate from NP40-lysate proteins reacting with chicken anti-gp17 antibodies. Analytical chromatography demonstrated that a number of gp17-like forms are present in the seminal plasma, put that only the 1 kDa species can be detected in the spermatozoa lysate. This protein was localised by immunofluorescence on the post-acrosomal region of the spermatozoon. The same surface domain was also reactive with anti-CD4 antibodies. After treatment to induce in vitro capacitation, gp17 was detected all over the spermatozoon head. Conversely, only a minor part of the treated spermatozoa exhibited CD4 immun...Continue Reading

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Citations

Jul 31, 2013·Journal of Assisted Reproduction and Genetics·Paula IntasquiRicardo P Bertolla
Jun 13, 2003·Fertility and Sterility·Andrea LasserreMónica H Vazquez-Levin
Nov 26, 2003·Journal of Reproductive Immunology·Will Wei-Cheng Chiu, Lawrence William Chamley
Apr 23, 1999·Journal of Reproductive Immunology·V L Scofield
Feb 19, 2008·Human Reproduction·Juan Martínez-HerediaRafael Oliva
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·N Dejucq, B Jégou
Nov 25, 2003·American Journal of Reproductive Immunology : AJRI·W W C Chiu, L W Chamley
Mar 18, 2015·Reproductive Biomedicine Online·Elisa GiacominiGiuseppe Ricci
Feb 9, 2019·Protein and Peptide Letters·Archana KumarKamini A Rao
Mar 14, 2000·The Journal of Biological Chemistry·E CaputoJ Guardiola
Aug 5, 2004·Journal of Andrology·Lena CarlssonAnders Larsson
Oct 31, 2014·Journal of Proteome Research·Christine LégaréRobert Sullivan
May 4, 2011·Fertility and Sterility·Stetson ThackerAshok Agarwal

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