CD4(+) regulatory T cells in gastric cancer mucosa are proliferating and express high levels of IL-10 but little TGF-β

Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
Bert KindlundSamuel B Lundin

Abstract

An increase of regulatory T cells, defined as CD25(high)- and/or FOXP3(+)-expressing CD4(+) T cells, within tumors has been reported in several studies. Tregs promote tumor growth by modulating the antitumor immune response, mainly through inhibition of T-cell-mediated tumor cell killing: this has been suggested to be dependent on IL-10 and/or TGF-β. In stomach cancer, the mechanisms behind the accumulation of Tregs in tumor tissue has not been fully elucidated, and neither has Treg gene expression in situ. Stomach tissue from gastric cancer patients undergoing gastric resection was analyzed using flow cytometry and cell sorting, followed by RT-PCR. We observed that stomach CD4(+) FOXP3(+) T cells proliferated to a higher degree than CD4(+) FOXP3(-) T cells, which may contribute to Treg accumulation in the mucosa. By analyzing DNA methylation, we demonstrated that both proliferating and nonproliferating FOXP3(+) T cells exhibited complete demethylation of the FOXP3 gene, indicating a stable FOXP3 expression in both cell populations. Furthermore, analysis of T-cell populations isolated directly from the tumor and tumor-free mucosa demonstrated that CD4(+) CD25(high) T cells have a higher IL-10/IFN-γ gene expression ratio but exp...Continue Reading

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Citations

Jun 1, 2016·Frontiers in Immunology·Jacobo López-AbenteMarjorie Pion
Sep 1, 2016·Journal of Cancer Research and Clinical Oncology·Wei-Ming ChenShu-Fen Wu
Mar 9, 2019·Molecular and Clinical Oncology·Xian SunJun-Feng Zhang
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Jun 12, 2020·Cell Communication and Signaling : CCS·Zhujiang DaiDaorong Wang
Aug 18, 2016·Helicobacter·Dominique VelinMarkus Gerhard
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Jun 4, 2020·Cellular Oncology (Dordrecht)·Łukasz ZadkaPiotr Dzięgiel
Nov 11, 2020·Biological Chemistry·Moein DehbashiWilliam C Cho
Feb 6, 2017·Cancer Immunology Research·Liu-Sheng PengYuan Zhuang
Feb 6, 2021·Biological Chemistry·Moein DehbashiWilliam C Cho
Jun 18, 2021·Frontiers in Oncology·Alissa Hendricks-WengerIrving Coy Allen
Jul 30, 2021·World Journal of Methodology·Masaya IwamuroHiroyuki Okada

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