CD4+ T-cell activation by antigen-presenting cells infected with urease-deficient recombinant Mycobacterium bovis bacillus Calmette-Guérin

FEMS Immunology and Medical Microbiology
Tetsu MukaiMasahiko Makino

Abstract

We constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG-Delta UT) that lacks urease, providing acidic intraphagosomal conditions to drive an effective human immune T-cell response. BCG-Delta UT-infected macrophages stimulated autologous CD4+ T cells more efficiently than parent BCG-infected macrophages. For further T-cell activation, BCG-Delta UT-infected macrophages required pretreatment with exogenous recombinant granulocyte-macrophage colony-stimulating factor or costimulation with either CD40 ligand or interferon-gamma. By contrast, BCG-Delta UT-infected dendritic cells induced significant activation of naïve CD4+ T cells without costimulating signals. C57BL/6 mice intradermally inoculated with BCG-Delta UT more efficiently produced memory T cells that responded to recall antigen. Therefore, the depletion of urease from BCG is useful for the activation of T cells.

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Citations

Dec 17, 2010·Clinical and Vaccine Immunology : CVI·Yumiko TsukamotoMasahiko Makino
May 17, 2014·PLoS Pathogens·Julian C Rutherford
Jun 10, 2010·FEMS Microbiology Letters·Daisuke HayashiKikuo Onozaki
Oct 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tetsu MukaiMasahiko Makino
Oct 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tetsu MukaiMasahiko Makino
Sep 28, 2012·Nihon Hansenbyō Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association·Masahiko Makino, Tetsu Mukai
Sep 29, 2020·Applied Biochemistry and Microbiology·N I NadolinskaiaA V Goncharenko
Mar 4, 2014·Nihon Hansenbyō Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association·Masahiko Makino

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