CD4 T Cell Affinity Diversity Is Equally Maintained during Acute and Chronic Infection

The Journal of Immunology : Official Journal of the American Association of Immunologists
Rakieb AndargachewBrian D Evavold

Abstract

TCR affinity for peptide MHC dictates the functional efficiency of T cells and their propensity to differentiate into effectors and form memory. However, in the context of chronic infections, it is unclear what the overall profile of TCR affinity for Ag is and if it differs from acute infections. Using the comprehensive affinity analysis provided by the two-dimensional micropipette adhesion frequency assay and the common indirect affinity evaluation methods of MHC class II tetramer and functional avidity, we tracked IAb GP61-80-specific cells in the mouse model of acute (Armstrong) and chronic (clone 13) lymphocytic choriomeningitis virus infection. In each response, we show CD4 T cell population affinity peaks at the effector phase and declines with memory. Of interest, the range and average relative two-dimensional affinity was equivalent between acute and chronic infection, indicating chronic Ag exposure did not skew TCR affinity. In contrast, functional and tetramer avidity measurements revealed divergent results and lacked a consistent correlation with TCR affinity. Our findings highlight that the immune system maintains a diverse range in TCR affinity even under the pressures of chronic Ag stimulation.

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Citations

Jul 6, 2019·Frontiers in Immunology·Shu-Wei WuZhengguo Xiao
Jun 9, 2020·Frontiers in Immunology·Nayan D Bhattacharyya, Carl G Feng
Oct 31, 2018·Frontiers in Immunology·Elizabeth M KolawoleBrian D Evavold
Jul 3, 2019·Trends in Immunology·Tanja LangeJürgen Westermann
Oct 31, 2020·International Journal of Molecular Sciences·Elizabeth M KolawoleBrian D Evavold
Mar 16, 2021·Frontiers in Immunology·Sachith D GunasingheKatharina Gaus

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