CD4 T cells specific for factor VIII are present at high frequency in healthy donors and comprise naïve and memory cells

Blood Advances
Sylvain MeunierBernard Maillère

Abstract

We investigated the frequency and subset origin of circulating factor VIII (FVIII)-specific CD4 T cells in healthy donors. Total CD4 T cells and purified CD4 T-cell subsets were stimulated with FVIII-loaded autologous dendritic cells and challenged for specificity in interferon-γ enzyme-linked immunospots. The number of specific T-cell lines allowed estimation of the frequency of T cells circulating in the blood of the donors. All the 16 healthy donors generated strong in vitro T-cell responses, leading to the generation of 154 FVIII-specific T-cell lines. The mean frequency of FVIII-specific CD4 T cells in healthy donors was similar to that of T cells specific for foreign antigens and greater than that of T cells specific for known immunogenic therapeutic proteins. Normal levels of endogenous FVIII in healthy donors therefore do not prevent a significant escape of FVIII-specific CD4 T cells from negative thymic selection. FVIII-specific T cells mainly originated from both the naïve and central memory cell subsets, but their frequencies remained low as compared with those of cells specific for foreign antigens in immunized donors. The observation of a spontaneous generation of FVIII-specific memory T cells without a global expa...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M AlgimanM D Kazatchkine
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·John J MarchalonisMichel D Kazatchkine
Jul 10, 2003·Autoimmunity Reviews·Sébastien Lacroix-DesmazesMichel D Kazatchkine
Aug 13, 2003·Journal of Thrombosis and Haemostasis : JTH·M T RedingB M Conti-Fine
Oct 3, 2003·Journal of Thrombosis and Haemostasis : JTH·G-L HuB M Conti-Fine
Mar 23, 2004·Annual Review of Immunology·Federica SallustoAntonio Lanzavecchia
May 11, 2010·Thrombosis Research·Saulius ButenasKenneth G Mann
Jun 12, 2010·Haemophilia : the Official Journal of the World Federation of Hemophilia·R A EttingerK P Pratt
Aug 25, 2010·Haemophilia : the Official Journal of the World Federation of Hemophilia·A KallasM Pooga
Mar 4, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephanie DellucBernard Maillere
Jul 27, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diletta Di MitriArne N Akbar
Feb 14, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·William W KwokJonathan H DeLong
May 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Florence Anne CastelliBernard Maillère
Sep 7, 2013·Thrombosis and Haemostasis·Massimo Franchini, Pier Mannuccio Mannucci
Sep 17, 2013·Nature Medicine·Gouri Shankar PandeyUNKNOWN PATH (Personalized Alternative Therapies for Hemophilia) Study Investigators
Jul 3, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claire ChevaleyreBernard Maillère
Jul 22, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·David M RichardsMarkus Feuerer

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Citations

May 2, 2020·Médecine sciences : M/S·Mélissa Bou JaoudehSébastien Lacroix-Desmazes
Mar 29, 2018·Blood·Richard S BlumbergUNKNOWN IgG Fc Immune Tolerance Group
Jul 2, 2020·Haemophilia : the Official Journal of the World Federation of Hemophilia·Kathleen P PrattSébastien Lacroix-Desmazes
Aug 15, 2020·Frontiers in Immunology·Sylvain MeunierBernard Maillère
Aug 4, 2019·The AAPS Journal·Osman N YogurtcuHong Yang
Jan 31, 2020·Frontiers in Immunology·Sebastien Lacroix-DesmazesKathleen P Pratt
Feb 6, 2020·Frontiers in Immunology·David W Scott, Kathleen P Pratt
May 19, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aurélien AzamBernard Maillère
Feb 18, 2020·British Journal of Haematology·Matthew CormierDavid Lillicrap
Jul 17, 2021·Frontiers in Immunology·Rami BecharaMarc Pallardy

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