CD4+ T helper cell responses against human bocavirus viral protein 2 viruslike particles in healthy adults

The Journal of Infectious Diseases
J LindnerS Modrow

Abstract

Human bocavirus (HBoV) was recently described as a new member of the Parvoviridae family, and its possible association with respiratory illness in infants has been discussed. To date, HBoV genomes have been detected worldwide in respiratory tract samples obtained from children with pulmonary diseases, whereas only limited data on virus-specific immunity are available, mainly because of the lack of recombinant viral antigens. HBoV viruslike particles (VLPs) were produced in insect cells and characterized by electron microscopy and cesium chloride gradient centrifugation. HBoV viral protein 2 (VP2)-specific antibodies and CD4+ T helper cell responses were analyzed by enzyme-linked immunosorbent assay and enzyme-linked immunospot assay. VP2 capsid proteins of HBoV were produced in insect cells infected with a recombinant baculovirus, and the formation of icosahedral VLPs (diameter, 21-25 nm; sedimentation density, 1.33 g/cm(3)) was demonstrated. A significant increase in secretion of VP2-specific interferon-gamma was detected in cultures of peripheral blood mononuclear cells obtained from 69 healthy adults found to be positive for HBoV-specific immunoglobulin G antibodies, compared with control stimulations. In parallel, T cell re...Continue Reading

References

Dec 1, 1995·The Journal of Infectious Diseases·M SöderlundK Hedman
Jul 1, 1996·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·A M Eis-HübingerK E Schneweis
Jan 19, 2002·Journal of Virology·Simone DorschSusanne Modrow
May 7, 2002·The Journal of Infectious Diseases·Martina SesterHans Köhler
Aug 26, 2003·Biologicals : Journal of the International Association of Biological Standardization·Nicola BoschettiChristoph Kempf
Sep 11, 2003·Biotechnology and Bioengineering·Luis MarangaManuel J T Carrondo
Jan 8, 2004·Journal of Hepatology·Daniel Shouval
Jan 22, 2005·Virology·Bärbel KaufmannRobert Seckler
Aug 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tobias AllanderBjörn Andersson
Nov 1, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Theo P SlootsIan M Mackay
Dec 1, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·T LowinS Modrow
Dec 1, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·J LindnerS Modrow
Mar 7, 2006·Journal of Clinical Microbiology·Xiaoming MaHideaki Kikuta
May 18, 2006·Emerging Infectious Diseases·Nathalie BastienYan Li
Oct 17, 2006·The Journal of Infectious Diseases·A ManningP Simmonds
Nov 1, 2006·Emerging Infectious Diseases·Nasser M KaplanC Anthony Hart
Dec 21, 2006·Emerging Infectious Diseases·Bernd KupferOliver Schildgen
Mar 6, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Fabrizio MaggiMauro Bendinelli
Mar 28, 2007·Journal of Medical Virology·Mohammadreza NaghipourC Anthony Hart
May 29, 2007·Virus Research·Thaweesak ChieochansinYong Poovorawan
Jun 8, 2007·Emerging Infectious Diseases·Diego VicenteEmilio Pérez-Trallero
Jul 27, 2007·Journal of Clinical Microbiology·Natalie BrieuVincent Foulongne
Aug 19, 2007·Journal of Clinical Microbiology·Rika EndoTadashi Ariga
Jan 18, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kalle KantolaMaria Söderlund-Venermo
Jun 10, 2008·Intervirology·Juha Lindner, Susanne Modrow
Oct 1, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Juha LindnerSusanne Modrow
Jan 1, 2009·Viral Immunology·Juha LindnerSusanne Modrow

❮ Previous
Next ❯

Citations

Feb 3, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Y WangJ Wang
Nov 5, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Juha LindnerSusanne Modrow
Apr 27, 2010·The Journal of Infectious Diseases·John V Williams
Dec 22, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Kai WangVincent Deubel
Dec 25, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Anita RiipinenMaria Söderlund-Venermo
May 29, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Martin EndersSusanne Modrow
Nov 1, 2011·Reviews in Medical Virology·Tuomas JarttiMaria Söderlund-Venermo
Feb 9, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M GuidoUNKNOWN Seroepidemiology Group
Apr 30, 2015·PloS One·Yexia HaoZhaojun Duan
Aug 24, 2012·PLoS Pathogens·Andrea MekkerUrs Karrer
Feb 20, 2016·Biomaterials·Nicholas M MolinoSzu-Wen Wang
Nov 4, 2016·Clinical Microbiology Reviews·Jianming QiuNeal S Young
Nov 8, 2016·World Journal of Gastroenterology : WJG·Marcello GuidoAntonella Zizza

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
FCS
transfection
Assay
FACS
electron microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Kalle KantolaMaria Söderlund-Venermo
Emerging Infectious Diseases
Diego VicenteEmilio Perez-Trallero
© 2022 Meta ULC. All rights reserved