CD40 expression by human peripheral blood eosinophils

The Journal of Clinical Investigation
Y OhkawaraM Jordana

Abstract

In this study, we have investigated CD40 expression in human peripheral blood eosinophils and in human chronically inflamed nasal tissues, i.e., nasal polyps. We show by both reverse transcriptase-PCR and Northern blot analysis that eosinophils from allergic subjects express human CD40 mRNA. We also show that constitutive CD40 mRNA expression in eosinophils could be upregulated by exposure to IgA immune complexes and downregulated by IL-10 and the synthetic steroid budesonide. In addition, we demonstrate that eosinophils express CD40 protein by flow cytometry. Such expression is biologically functional as cross-linking CD40 with CD40 mAbs enhances eosinophil survival in a dose-dependent fashion; in addition, CD40 ligation stimulates eosinophils to release GM-CSF. CD40-mediated eosinophil survival was largely inhibited by an anti-GM-CSF neutralizing antibody suggesting GM-CSF involvement in the survival enhancing mechanism. CD40 mRNA was also detected in total RNA extracted from nasal polyp tissues but not in RNA isolated from normal nasal mucosa (inferior turbinate); by immunohistochemistry, we were able to detect immunoreactive CD40 protein in a variety of cell types in the polyp stroma, but primarily in eosinophils. These obs...Continue Reading

References

Dec 1, 1992·European Journal of Immunology·D GrafR A Kroczek
Dec 1, 1992·The Journal of Experimental Medicine·M K SpriggsW C Fanslow
Jul 1, 1990·Tissue Antigens·E A Clark
Jan 1, 1990·International Archives of Allergy and Applied Immunology·P ValentP Bettelheim
Jun 1, 1991·American Journal of Respiratory Cell and Molecular Biology·G CoxM Jordana
Dec 21, 1989·Nature·Y J LiuI C MacLennan
Jan 1, 1985·Cancer Immunology, Immunotherapy : CII·S PaulieP Perlmann
Oct 1, 1994·The Journal of Experimental Medicine·C CauxJ Banchereau
Jul 1, 1995·The Journal of Experimental Medicine·D HollenbaughA Aruffo
Aug 1, 1993·The Journal of Experimental Medicine·M R AldersonM K Spriggs
Mar 1, 1995·European Journal of Immunology·J F GauchatD Aldebert
Feb 1, 1994·The Journal of Experimental Medicine·S DubucquoiM Capron

❮ Previous
Next ❯

Citations

Mar 29, 2006·Current Allergy and Asthma Reports·Flurina MeilerMübeccel Akdis
Jun 1, 1997·Immunology Today·M Pretolani, M Goldman
Jan 1, 1997·Research in Immunology·M Pretolani, M Goldman
Jan 20, 1998·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·I LalaniA R Ahmed
Sep 21, 2001·Microbes and Infection·C A Akdis, K Blaser
Sep 21, 2001·Microbes and Infection·H YsselJ Pène
Sep 5, 2002·Respiratory Physiology & Neurobiology·Nobuhisa IwaseYoshio Maruyama
Jun 20, 2000·Allergy·C A Akdis, K Blaser
Jan 13, 2010·Immunology and Cell Biology·Lisa A Spencer, Peter F Weller
Sep 26, 2006·Nature Reviews. Immunology·Mark LarchéRudolf Valenta
Jun 20, 2001·Immunology·C A Akdis, K Blaser
Mar 1, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·G KrishnaswamyR Stout
Dec 2, 2009·Current Opinion in Allergy and Clinical Immunology·Praveen AkuthotaPeter F Weller
Nov 8, 2012·Current Opinion in Gastroenterology·Simon P HoganPatricia C Fulkerson
Aug 3, 1999·American Journal of Respiratory and Critical Care Medicine·G M GauvreauP M O'Byrne
Apr 27, 2000·American Journal of Respiratory Cell and Molecular Biology·T TamadaK Shirato
Oct 6, 2000·American Journal of Respiratory Cell and Molecular Biology·Y OhkawaraK Shirato
Apr 29, 1998·The Journal of Clinical Investigation·X F LeiM Jordana
Jul 3, 1998·The Journal of Clinical Investigation·C A AkdisK Blaser
Apr 5, 2000·The Journal of Clinical Investigation·H Z ShiP F Weller
Jun 18, 2005·Memórias do Instituto Oswaldo Cruz·Christianne Bandeira-Melo, Peter F Weller
Oct 22, 2008·Allergologia et immunopathologia·A J Lucendo
Nov 19, 2008·The Journal of Allergy and Clinical Immunology·John T Kelly, William W Busse
May 17, 2006·Immunology and Allergy Clinics of North America·Johan VerhagenMübeccel Akdis
Nov 27, 2008·Clinical and Experimental Immunology·I FenoyA Goldman
Aug 30, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P AkuthotaP F Weller
Mar 27, 2009·Veterinary Dermatology·Christine Loewenstein, Ralf S Mueller
Aug 24, 2010·Immunological Reviews·John P GrahamGail A Bishop
Aug 12, 2014·Microbes and Infection·Bao-Ping TianSongmin Ying
Jan 23, 2010·Allergology International : Official Journal of the Japanese Society of Allergology·Takashi Fujimura, Yoshitaka Okamoto
Mar 4, 2014·The Journal of Allergy and Clinical Immunology·Mübeccel Akdis, Cezmi A Akdis
Jan 1, 1997·Memórias do Instituto Oswaldo Cruz·P Lacy, R Moqbel
Jan 1, 1997·Memórias do Instituto Oswaldo Cruz·P F Weller, K Lim
Nov 24, 1999·Critical Reviews in Clinical Laboratory Sciences·G M Walsh
Jun 5, 2002·Current Opinion in Allergy and Clinical Immunology·Simon P HoganMarc E Rothenberg
Mar 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K Blaser, C A Akdis
Mar 12, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amali E SamarasingheJonathan A McCullers

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