CD40 ligand (CD154) incorporated into HIV virions induces activation-induced cytidine deaminase (AID) expression in human B lymphocytes.

PloS One
Marta EpeldeguiOtoniel Martínez-Maza

Abstract

Most AIDS-associated non-Hodgkin's lymphoma (AIDS-NHL) arises from errors in immunoglobulin heavy-chain gene (IgH) class switch recombination (CSR) or somatic hypermutation (SHM), events that occur in germinal center (GC) B cells and require the activity of activation induced cytidine deaminase (AID). Several oncogenic viruses (EBV, HCV, HPV) can induce AID gene (AID) expression, and elevated AID expression is seen in circulating lymphocytes prior to AIDS-NHL diagnosis. Here, we report that HIV produced in peripheral blood mononuclear cells (PBMC) induced AID expression in normal human B cells. Since HIV produced in PBMC contains host cell CD40 ligand (CD40L) incorporated into the viral membrane, and CD40L is known to induce AID expression in human B cells, the role of virion-associated CD40L in HIV-induced AID expression was examined. Only viruses expressing functional CD40L were seen to induce AID expression; CD40L-negative HIV did not induce AID expression. The induction of AID expression by CD40L+ HIV was abrogated by addition of blocking anti-CD40L antibody. AID protein was detected in B cells exposed to CD40L+ HIV using intracellular multicolor flow cytometry, with most AID producing B cells expressing the CD71 activation...Continue Reading

References

Sep 1, 1992·Research in Immunology·O Martínez-Maza
Oct 1, 1990·Immunology Today·A Amadori, L Chieco-Bianchi
Jun 1, 1983·Clinical Immunology and Immunopathology·R W SchroffJ L Fahey
Jun 1, 1993·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J M PludaR Yarchoan
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·J ReiserM Schubert
Jun 5, 2002·Nature Reviews. Cancer·Chris Boshoff, Robin Weiss
Aug 3, 2002·Nature Immunology·Mikhail B LitinskiyAndrea Cerutti
Aug 23, 2002·Current Opinion in Oncology·Otoniel Martínez-Maza, Elizabeth Crabb Breen
Nov 5, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Elizabeth Crabb BreenOtoniel Martínez-Maza
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bing HeAndrea Cerutti
Mar 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Keigo MachidaMichael M C Lai
Aug 19, 2004·Cell·Almudena R RamiroMichel C Nussenzweig
Jan 5, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fabrice BonnetUNKNOWN Groupe d'Epidemiologie Clinique du SIDA en Aquitaine
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Angela MalaspinaAnthony S Fauci
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bing HeAndrea Cerutti
May 3, 2006·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Elizabeth Crabb BreenOtoniel Martínez-Maza
Aug 9, 2006·Current Opinion in Oncology·Marta EpeldeguiOtoniel Martínez-Maza
Jul 10, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Bradley A CicciarelliKenneth A Smith
Aug 29, 2007·The Journal of Experimental Medicine·Yair DorsettMichel C Nussenzweig
Dec 11, 2007·Nature Genetics·Laura PasqualucciRiccardo Dalla-Favera
Apr 1, 2008·Radiation Protection Dosimetry·Jozef RosinaDana Drábová
Jul 16, 2008·Cell Host & Microbe·Simon SwinglerMario Stevenson

❮ Previous
Next ❯

Citations

Feb 14, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chad J AchenbachUNKNOWN Centers for AIDS Research (CFAR) Network of Integrated Clinical Systems (CNICS)
Aug 19, 2010·Immunologic Research·Marta EpeldeguiOtoniel Martínez-Maza
Oct 5, 2010·Journal of Proteome Research·Elena Vendrame, Otoniel Martínez-Maza
Oct 28, 2010·Current Opinion in HIV and AIDS·Richard F AmbinderRonald Mitsuyasu
Dec 17, 2010·Current HIV/AIDS Reports·Xiaoying Shen, Georgia D Tomaras
Apr 30, 2011·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Elizabeth Crabb BreenOtoniel Martinez-Maza
Jun 1, 2013·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Yu GuoOtoniel Martínez-Maza
Aug 30, 2013·Retrovirology·Sabrina CurreliJoseph Bryant
Jul 29, 2015·Critical Reviews in Clinical Laboratory Sciences·Ravnit GrewalCiprian Tomuleasa
Mar 13, 2016·Seminars in Oncology·Priscila H GoncalvesThomas S Uldrick
Jun 29, 2012·Cytokine & Growth Factor Reviews·Chao Wang, Tania H Watts
Jun 13, 2012·Seminars in Immunology·Alexandre Orthwein, Javier M Di Noia
Dec 9, 2014·The Journal of Clinical Investigation·Shi-Dong MaShannon C Kenney
May 5, 2017·Journal of Virology·Supranee BuranapraditkunHendrik Streeck
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Dongsheng LiDavid Harrich
Dec 12, 2018·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Gordana HalecShehnaz K Hussain
Jun 30, 2019·Scientific Reports·Marta EpeldeguiOtoniel Martínez-Maza
Aug 11, 2020·Journal of Cancer Research and Clinical Oncology·Leonardo Alves de Souza RiosShaheen Mowla
May 18, 2021·Frontiers in Oncology·Pedro S de CarvalhoMarcelo A Soares
Jun 17, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Laura E MartínezMarta Epeldegui

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
ELISA
PCR

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.