CD40 ligand expression in mycobacterium bovis BCG infection and its regulation by cytokines: a direct role of interleukin 12

Archives of Medical Research
P Méndez-Samperio, E García-Martínez

Abstract

Activation and clonal expansion of T cells require not only the recognition of processed antigen on the surface of the antigen-presenting cell (APC) by T-cell receptor (TCR), but also involve co-stimulatory signals that are provided by the simultaneous engagement of cell surface molecules expressed by both the APC and the T cell. Interaction between CD40 and its ligand (CD40L) is known to mediate host immune response and T-cell-mediated effector functions in mycobacterial infections in mice. In this work, we investigated the capacity of Mycobacterium bovis (M. bovis) BCG to induce the expression of CD40L on human T cells. Human cells were obtained from healthy adults by centrifugation using Ficoll/Hypaque. Cells (1 x 10(6)) were incubated in RPMI medium with BCG. After incubation at 37 degrees C in 5% CO(2) atmosphere for 40 h, cells were collected and double-stained with anti-CD40L-PE and anti-CD4-FITC or anti-CD8-FITC mAb. The quantification of positively stained population was based on samples stained with isotype control antibodies analyzed on a FACScan. M. bovis BCG stimulation induced a significant amount of CD40L expression on CD4+ T cells, while CD40L was not significantly detected on human CD8+ T cells. The highest CD4...Continue Reading

References

Aug 1, 1995·Current Opinion in Immunology·A M Cooper, J L Flynn
Jul 1, 1995·The Journal of Experimental Medicine·D HollenbaughA Aruffo
Aug 1, 1993·The Journal of Experimental Medicine·M R AldersonM K Spriggs
Jan 1, 1993·Annual Review of Immunology·P S Linsley, J A Ledbetter

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Citations

Aug 26, 2009·Journal of Child and Adolescent Psychiatric Nursing : Official Publication of the Association of Child and Adolescent Psychiatric Nurses, Inc·Scott Weber

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