PMID: 7542511Jan 1, 1994Paper

CD44 in differentiated embryonic stem cells: surface expression and transcripts encoding multiple variants

Developmental Immunology
H HaegelR Ceredig

Abstract

Expression of the surface-adhesion molecule CD44 was investigated during the in vitro differentiation of the embryonic stem (ES) cell line D3. By immunofluorescence analysis, totipotent, undifferentiated ES cells did not show surface expression of CD44, although two transcripts of approximately 1.6 and 3.3 kb were detected on Northern blots. Following 1 week of differentiation in either suspension or substrate-attached cultures, CD44 appeared on the surface of some D3 cells, and synthesis of an additional 4.5 kb mRNA species was detected on Northern blots. At this stage, at least three distinct transcripts encoding CD44 variants were induced within the cultures, resulting from alternative splicing of additional exons in the variable domains of CD44. From PCR analysis, they all appeared to contain the variable exon v10, and two of them in addition contained v6. Taken together, these results suggest that CD44 may play a role in cell migration and adhesion in the early development of the mouse embryo.

Citations

May 3, 2013·In Vitro Cellular & Developmental Biology. Animal·Chiann-Chyi ChenYacov Ron
Oct 29, 2003·Theriogenology·Cecilia C FurnusAna Lía Errecalde
Mar 25, 2000·Theriogenology·C C FurnusA G Martínez
Jul 1, 1996·Tissue Engineering·J H Brekke
Jun 13, 2015·Oxidative Medicine and Cellular Longevity·Ilaria DandoMarta Palmieri
May 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xiaochen ZhouYi Sun
Feb 24, 2012·Biology of Reproduction·Hiroko MorimotoTakashi Shinohara
Apr 20, 2013·Experimental Biology and Medicine·Karin WilliamsSusan Kasper
Dec 17, 1998·Journal of Biomedical Materials Research·J H Brekke, J M Toth
Mar 1, 2019·Chembiochem : a European Journal of Chemical Biology·Hiroyuki ShinchiYasuo Suda
Jun 12, 2019·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Z D Sahin Inan, S Unver Saraydin
Oct 23, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eun-Jin YunJer-Tsong Hsieh

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