CD45 up-regulation during lymphocyte maturation

International Immunology
J Kirberg, T Brocker

Abstract

CD4+ CD8+ double-positive thymocytes differentiate into CD4+ and CD8+ single-positive T cells during thymic positive selection. This process requires the interaction between the TCR and self MHC molecules. In this context we have analyzed the expression of CD45, an abundant transmembrane protein tyrosine phosphatase, and describe here its differential surface expression during T cell maturation. Using four-color FACS analysis of thymocytes from normal as well as TCR-transgenic mice we demonstrate that CD45 is up-regulated only during positive selection concomitantly with the TCR-CD3 complex and the transient early activation marker CD69, but that this up-regulation precedes heat stable antigen down-regulation. The tight linkage of the up-regulation of the TCR-CD3 complex and CD45 may be required because the CD45 tyrosine phosphatase plays a role in modulating signal transduction by the TCR-CD3 complex during positive selection. In addition, our findings argue for a regulation mechanism that adapts the CD45 levels to increasing antigen receptor levels on mature T cells and B cells.

Citations

Jan 16, 1998·Immunology and Cell Biology·J G Altin, E K Sloan
Jul 23, 2014·European Journal of Clinical Investigation·Ulrich GrabmaierStefan Brunner
Nov 5, 2013·Clinical & Developmental Immunology·Xi XuQing Ge
Sep 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Duncheng WangMichele K Anderson
Jun 10, 2020·Diagnostics·Giovanni SmaldoneMarco Salvatore

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