Abstract
CD45RA T cells are fully co-activated by natural beta1 integrin ligands fibronectin (FN) and VCAM-1, as well as monoclonal antibody (mAb) 19H8, which binds a combinatorial epitope of the alpha4beta1 heterodimer. These integrin ligands stimulate CD3-dependent proliferation and the upregulation of early activation markers CD25 and CD69. However, beta1-specific antibody 33B6, which binds to a similar range of the predominant T-cell integrins as natural ligands FN (alpha4beta1 and alpha5beta1) and VCAM-1 (alpha4beta1), failed to costimulate proliferation in the CD45RA subset, while retaining the ability to costimulate early activation markers CD25 and CD69. After addition of exogenous human interleukin-2 to the culture media, 33B6 costimulation of proliferation is restored. These data provide evidence that a branch of the alpha4beta1 integrin-signaling pathway in CD45RA T cells can be independently regulated and exploited through the use of partial agonist ligands, including mAbs to the integrin heterodimer.
References
Mar 5, 1992·Nature·T A YednockN Karin
Feb 23, 1990·Cell·M J ElicesR R Lobb
Nov 1, 1991·European Journal of Immunology·L C BurklyR R Lobb
Aug 1, 1991·The Journal of Clinical Investigation·A LaffónF Sánchez-Madrid
Oct 1, 1990·The Journal of Experimental Medicine·Y NojimaC Morimoto
Jun 15, 1990·Science·R H Schwartz
Jan 12, 1990·Cell·J L Guan, R O Hynes
Feb 7, 1987·Journal of Theoretical Biology·R J de Boer, P Hogeweg
Jul 1, 1994·Immunology Today·C H JuneC B Thompson
Aug 1, 1995·The Journal of Experimental Medicine·P AltevogtE C Butcher
Dec 1, 1994·Cell Adhesion and Communication·T K TeagueB W McIntyre
Jan 1, 1994·Annual Review of Immunology·I S Trowbridge, M L Thomas
Jan 1, 1993·The Journal of Experimental Medicine·E EnnisY Shimizu
Jun 17, 1998·Molecular Biology of the Cell·S DendaU Müller
Jun 24, 1998·Transplantation Proceedings·A J CoitoJ W Kupiec-Weglinski
Jul 10, 1999·Science·A GrakouiM L Dustin
Aug 17, 1999·Neurology·N TubridyD H Miller
Aug 24, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·D G WoodsideB W McIntyre
Sep 13, 2003·Immunology and Cell Biology·Andrew C IssekutzThomas B Issekutz
Sep 23, 2003·Science·Minsoo KimTimothy A Springer
May 5, 2004·The Journal of Biological Chemistry·Bing-Hao LuoJunichi Takagi
Jul 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·María MittelbrunnFrancisco Sánchez-Madrid
Oct 8, 2005·The Journal of Allergy and Clinical Immunology·Virginia NorrisStephane Kirkesseli
Jan 21, 2006·Immunology·Nick Holmes
Jan 21, 2006·Trends in Immunology·Elma Z Tchilian, Peter C L Beverley
Aug 18, 2006·Current Opinion in Neurology·Russell E Bartt
Citations
Jul 6, 2010·Neural Networks : the Official Journal of the International Neural Network Society·Nathan KoenigMaja Matarić
Oct 10, 2009·Human Immunology·Tae Kon KimKrishna V Komanduri
Jun 14, 2013·The Biochemical Journal·Ann-Marie CimoJohn E Ladbury
Mar 11, 2009·Journal of Immunological Methods·Christina L LancioniRoxana E Rojas
Jul 1, 2017·International Journal of Molecular Sciences·Thiago Aparecido da SilvaMaria Cristina Roque-Barreira
Feb 24, 2011·The Journal of Biological Chemistry·Sukhbir KaurDavid D Roberts