CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model

Arthritis Research & Therapy
Mahmoud OrazizadehDonald M Salter

Abstract

Recent studies provide evidence of roles for integrins in mechanical signalling in bone and cartilage. Integrin signalling is modulated by various mechanisms, including interaction with other transmembrane proteins. We aimed to identify whether one such protein, integrin-associated protein (CD47/IAP), is expressed by chondrocytes and whether it may regulate integrin-dependent mechanotransduction. Chondrocytes, isolated from macroscopically normal and osteoarthritic articular cartilage of human knee joints, were studied in a resting state or following mechanical stimulation at 0.33 Hz. CD47/IAP expression and associations were confirmed by immunohistology, reverse transcription-polymerase chain reaction, Western blotting, and immunoprecipitation. Roles in mechanotransduction were studied by assessing effects of function-blocking antibodies on a range of electrophysiological, cellular, and molecular responses of primary chondrocytes and responses of CD47/IAP-null cell lines transfected with CD47/IAP. Human articular chondrocytes were shown to express CD47/IAP, predominantly the type 2 isoform. Immunoprecipitation showed association of CD47/IAP with alpha5 integrin and thrombospondin but not SIRPalpha (signal-regulatory protein-al...Continue Reading

References

Jan 1, 1989·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R L SahJ D Sandy
Oct 1, 1996·The Journal of Cell Biology·A G GaoW A Frazier
Mar 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D A Lee, D L Bader
Jun 6, 1997·The Journal of Biological Chemistry·J ChungW A Frazier
Jul 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D M SalterM O Wright
Jan 8, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M O WrightD M Salter
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J N TopperM A Gimbrone
Mar 20, 1999·The Journal of Biological Chemistry·W A FrazierM E Linder
Jun 2, 2000·Annals of the Rheumatic Diseases·D PfanderB Swoboda
Aug 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H S LeeD M Salter
Aug 15, 2000·European Journal of Immunology·E F Vernon-WilsonM H Brown
Feb 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G JinS Chien
Apr 18, 2001·Trends in Cell Biology·E J Brown, W A Frazier
May 25, 2002·Molecular and Cellular Biology·Liz SvenssonAke Oldberg
Apr 12, 2003·The Journal of Biological Chemistry·Laurence LamyAlain Bernard
Feb 12, 2004·Cancer Research·Partha Pratim Manna, William A Frazier
Dec 28, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Damian C GenetosRandall L Duncan

❮ Previous
Next ❯

Citations

Jun 28, 2008·Current Opinion in Cell Biology·Martin A Schwartz, Douglas W DeSimone
Jun 23, 2009·Scandinavian Journal of Medicine & Science in Sports·L RamageD M Salter
Mar 29, 2014·Birth Defects Research. Part C, Embryo Today : Reviews·Denitsa DochevaAttila Aszodi
Aug 30, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Richard F Loeser
Sep 28, 2014·Rheumatology International·Jian TianGuang-Hua Lei
Sep 6, 2019·Cellular Oncology (Dordrecht)·Seyed Mohammad Gheibi HayatAmirhossein Sahebkar
Jul 18, 2020·Journal of Hematology & Oncology·Entsar EladlHong Chang
Apr 3, 2020·Journal of Cellular and Molecular Medicine·Zhenxing ZhaoJie Fang
Jul 6, 2021·Frontiers in Cell and Developmental Biology·Hongfu JinYusheng Li

❮ Previous
Next ❯

Methods Mentioned

BETA
amputations
immunoprecipitation
PCR
electrophoresis
transfection
GTPases
GTPase

Software Mentioned

EASY Image

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.