PMID: 7515097Jun 1, 1994Paper

CD50 (intercellular adhesion molecule 3) stimulation induces calcium mobilization and tyrosine phosphorylation through p59fyn and p56lck in Jurkat T cell line

The Journal of Experimental Medicine
M JuanJ Vives

Abstract

The leukocyte differentiation antigen, CD50, has been recently identified as the intercellular adhesion molecule 3 (ICAM-3), the third counter-receptor of leukocyte function-associated antigen 1 (LFA-1). This molecule seems to be specially involved in the adhesion events of the initial phases of the immune response. To characterize the role of CD50 in leukocyte interactions, the different molecular events induced after cross-linking of CD50 on T cell-derived Jurkat cell line have been analyzed. When cells were incubated with anti-CD50 mAbs and cross-linked with polyclonal goat anti-mouse immunoglobulins, a rise in intracellular calcium concentration ([Ca2+]i) was observed. This increase in [Ca2+]i was mainly due to the uptake of extracellular Ca2+. This Ca2+ flux involved tyrosine phosphorylations and was further increased by CD3 costimulation. These data, together with those obtained by phosphotyrosine (P-Tyr) immunoprecipitation and in vitro kinase assays, suggested the involvement of protein-tyrosine kinases (PTK) in CD50 transduction pathways. By using specific antisera, the presence of p56lck and p59fyn protein tyrosine kinases (PTK) was clearly demonstrated in the CD50 immunoprecipitates. These findings suggest that the i...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A D BeyersA F Williams
Jul 1, 1991·The Journal of Experimental Medicine·A R de FougerollesT A Springer
Oct 24, 1990·European Journal of Biochemistry·F LozanoJ Vives
Nov 1, 1990·Tissue Antigens·R VilellaJ Vives
Aug 2, 1990·Nature·T A Springer
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L E SamelsonR D Klausner
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C H JuneL E Samelson
Aug 1, 1989·The Journal of Experimental Medicine·M C WacholtzP E Lipsky
Oct 1, 1987·European Journal of Immunology·J MartorellJ Vives
Jul 1, 1993·European Journal of Immunology·M JuanA R De Fougerolles
Jun 1, 1993·Current Opinion in Immunology·B Malissen, A M Schmitt-Verhulst

❮ Previous
Next ❯

Citations

Jan 1, 1996·Molecular Reproduction and Development·P Olds-ClarkeG S Kopf
Feb 17, 2001·European Journal of Biochemistry·O FunatsuK Furukawa
May 6, 2005·Molecular Biology of the Cell·Hortensia de la FuenteFrancisco Sánchez-Madrid
Jan 5, 2013·Human Reproduction Update·Xiang XiaoC Yan Cheng
Nov 28, 1997·American Journal of Respiratory Cell and Molecular Biology·T H KalbL Mayer
Jan 29, 2008·Blood·Li TianCarl G Gahmberg
Sep 18, 2002·Immunological Reviews·María C MontoyaFrancisco Sánchez-Madrid
Apr 21, 1997·The Journal of Cell Biology·M A del PozoF Sánchez-Madrid
Feb 4, 1999·Cell Adhesion and Communication·S López-BrionesR González-Amaro
Nov 28, 2009·Immunology Letters·Judith LeitnerPeter Steinberger
Jan 29, 2002·Nature Immunology·María C MontoyaFrancisco Sánchez-Madrid
Apr 26, 2001·Journal of Physiology and Biochemistry·J RiveraV Vicente
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C D O'BrienS M Albelda
Jun 1, 1995·Annals of Medicine·R Busse, I Fleming
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A L LazaarS K Das
May 10, 2005·International Journal of Cancer. Journal International Du Cancer·Young Min ChungDuk-Soo Bae
Feb 2, 1996·The Journal of Biological Chemistry·T E TaherS T Pals

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.