CD63 inhibits the cell migration and invasion ability of tongue squamous cell carcinoma

Oncology Letters
Wen-Hui LiuWei Zhao

Abstract

The present study evaluated the expression and potential role of CD63 in the migration and invasion of tongue squamous cell carcinoma (TSCC) cells. Immunohistochemistry (IHC) was used to investigate the association between the expression level of CD63 protein and the histological differentiation of samples from 40 patients with TSCC and four normal tongue tissue specimens. RNA interference (RNAi) and gene transfection technology were used to alter the expression of CD63 in TCA8113 cells. The stable silencing and overexpression of CD63 in the TCA8113 cell line was used to assess the impact of the CD63 expression level on the migratory and invasive abilities of TCA8113 cells in a wound healing assay and a Transwell invasion assay. The effect of CD63 on the expression of matrix metalloproteinase (MMP)-2 and -9 were evaluated by western blot analysis. The results of IHC revealed a positive association between the CD63 expression level and the histopathological differentiation of TSCC and a negative association between the CD63 expression level and lymph node metastasis in TSCC. Western blotting revealed that the expressions of MMP-2 and MMP-9 were clearly upregulated in CD63-silenced TCA8113 cells but reduced in CD63-overexpressing...Continue Reading

Citations

Dec 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhenguo SongTao Wang

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Methods Mentioned

BETA
transfection
Protein Assay
PCR
electrophoresis
confocal microscopy

Software Mentioned

ImageJ1
Image Analysis System
Omega Lum [UNK] G capture
SPSS Statistics
Omega Lum G

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