CD66-mediated phagocytosis of Opa52 Neisseria gonorrhoeae requires a Src-like tyrosine kinase- and Rac1-dependent signalling pathway

The EMBO Journal
Christof R HauckE Gulbins

Abstract

The interaction of Neisseria gonorrhoeae with human phagocytes is a hallmark of gonococcal infections. Recently, CD66 molecules have been characterized as receptors for Opa52-expressing gonococci on human neutrophils. Here we show that Opa52-expressing gonococci or Escherichia coli or F(ab) fragments directed against CD66, respectively, activate a signalling cascade from CD66 via Src-like protein tyrosine kinases, Rac1 and PAK to Jun-N-terminal kinase. The induced signal is distinct from Fcgamma-receptor-mediated signalling and is specific for Opa52, since piliated Opa- gonococci, commensal Neisseria cinerea or E.coli do not stimulate this signalling pathway. Inhibition of Src-like kinases or Rac1 prevents the uptake of Opa52 bacteria, demonstrating the crucial role of this signalling cascade for the opsonin-independent, Opa52/CD66-mediated phagocytosis of pathogenic Neisseria.

References

Dec 1, 1992·Trends in Genetics : TIG·B D Robertson, T F Meyer
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L WeinsteinA L DeFranco
Jan 1, 1991·Journal of Clinical Laboratory Analysis·J A ThompsonW Zimmermann
Jan 1, 1989·Annual Review of Microbiology·W M Shafer, R F Rest
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R HaasT F Meyer
Dec 1, 1988·The Journal of Experimental Medicine·J SwansonJ Boslego
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T F MeyerM So
Nov 24, 1995·The Journal of Biological Chemistry·S BagrodiaR A Cerione
Jun 1, 1995·The International Journal of Biochemistry & Cell Biology·P KefalasP M Brickell
Aug 1, 1995·The Journal of Experimental Medicine·T ChenJ Swanson
Jan 1, 1994·Annual Review of Cell Biology·A Hall
Jun 1, 1993·Current Opinion in Immunology·D Brown
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G M Twamley-SteinS A Courtneidge
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·S GreenbergB Seed
Mar 1, 1996·Leukemia Research·Y XuC L Willman
Feb 1, 1996·Current Opinion in Cell Biology·S H Zigmond
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Chen, E C Gotschlich
Jan 20, 1997·The Journal of Experimental Medicine·M WataraiC Sasakawa
Mar 1, 1997·Current Biology : CB·M A SellsJ Chernoff

❮ Previous
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Citations

Jun 22, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K Kwiatkowska, A Sobota
Jun 22, 1999·Immunologic Research·K M Coggeshall
Oct 26, 1999·Cellular Signalling·G Berton, C A Lowell
Jan 16, 2002·Current Opinion in Immunology·Steven Greenberg, Sergio Grinstein
Apr 30, 1998·Current Opinion in Cell Biology·K Ireton, P Cossart
May 14, 2003·Trends in Cell Biology·Patrice Boquet, Emmanuel Lemichez
Mar 5, 2003·Current Opinion in Microbiology·Christof R Hauck, Thomas F Meyer
Feb 27, 1999·Current Opinion in Microbiology·M NaumannT F Meyer
Sep 6, 2012·The Journal of Biological Chemistry·Kathrin KoppChristof R Hauck
Jun 6, 2000·The Journal of Experimental Medicine·T SuzukiC Sasakawa
Jan 7, 2004·The Journal of Experimental Medicine·Phillip Henneke, Douglas T Golenbock
Jan 23, 1999·Current Opinion in Hematology·G Berton
Oct 19, 2004·Clinical Microbiology Reviews·Jennifer L Edwards, Michael A Apicella
Apr 16, 2005·Clinical Microbiology Reviews·Alain L Servin
Jun 23, 2006·Infection and Immunity·Christiane KühleweinThomas Rudel
Sep 13, 2001·Infection and Immunity·A RytkönenA B Jonsson
Jan 19, 1999·Annual Review of Cell and Developmental Biology·S Dramsi, P Cossart
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A J Merz, M So
Dec 17, 2008·Journal of Translational Medicine·Keith M Skubitz, Amy P N Skubitz

❮ Previous
Next ❯

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