PMID: 8976201Dec 1, 1996Paper

CD8 beta increases CD8 coreceptor function and participation in TCR-ligand binding

The Journal of Experimental Medicine
V RenardI F Luescher

Abstract

To study the role of CD8 beta in T cell function, we derived a CD8 alpha/beta-(CD8-/-) T cell hybridoma of the H-2Kd-restricted N9 cytotoxic T lymphocyte clone specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide PbCS 252-260. This hybridoma was transfected either with CD8 alpha alone or together with CD8 beta. All three hybridomas released interleukin 2 upon incubation with L cells expressing Kd-peptide derivative complexes, though CD8 alpha/beta cells did so more efficiently than CD8 alpha/alpha and especially CD8-/- cells. More strikingly, only CD8 alpha/beta cells were able to recognize a weak agonist peptide derivative variant. This recognition was abolished by Fab' fragments of the anti-Kd alpha 3 monoclonal antibody SF1-1.1.1 or substitution of Kd D-227 with K, both conditions known to impair CD8 coreceptor function. T cell receptor (TCR) photoaffinity labeling indicated that TCR-ligand binding on CD8 alpha/beta cells was approximately 5- and 20-fold more avid than on CD8 alpha/a and CD8-/- cells, respectively. SF1-1.1.1 Fab' or Kd mutation D227K reduced the TCR photoaffinity labeling on CD8 alpha/beta cells to approximately the same low levels observed on CD8-/- cells. These results...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A D BeyersA F Williams
May 21, 1992·Nature·C J WheelerJ R Parnes
Apr 1, 1995·The Journal of Experimental Medicine·H Y IrieS J Burakoff
Jan 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J Leahy

❮ Previous
Next ❯

Citations

Dec 18, 2001·European Journal of Immunology·T KambayashiH G Ljunggren
Sep 15, 2005·Journal of Clinical Immunology·Claude LambertGenin Christian
Jan 16, 2002·Current Opinion in Immunology·Rolf König
Feb 15, 2008·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·C BidotC Lambert
Dec 18, 2008·International Immunology·Georgi S AngelovImmanuel F Luescher
May 23, 1998·Annual Review of Immunology·M M DavisY Chien
Oct 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·SangKon OhJay A Berzofsky
Mar 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Raphaël GenoletImmanuel F Luescher
Dec 31, 1997·The Journal of Experimental Medicine·A ViolaA Lanzavecchia
Aug 22, 2003·Clinical and Experimental Immunology·S WerwitzkeT Witte
May 9, 2007·Immunology·Charles J Kroger, Martha A Alexander-Miller
Apr 15, 2008·Scandinavian Journal of Immunology·S WerwitzkeT Witte
Apr 9, 2013·PloS One·Rosario CastroPierre Boudinot
Nov 6, 2007·Fish & Shellfish Immunology·Xiao-Feng SunQiong-Lin Guo
May 12, 2004·Current Opinion in Immunology·Denise Gangadharan, Hilde Cheroutre
Dec 2, 2009·European Journal of Immunology·Yoon-Joong KangHsiu-Ching Chang
Apr 12, 2003·The Journal of Biological Chemistry·Marie-Agnès DouceyImmanuel F Luescher
Apr 17, 2003·The Journal of Biological Chemistry·Sarah L HutchinsonAndrew K Sewell
Jul 15, 2006·The Journal of Biological Chemistry·Hsiu-Ching ChangYen-Ming Hsu
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A ArcaroI F Luescher
Feb 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mythili KoneruAlan B Frey
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charles J KrogerMartha A Alexander-Miller
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charlly KaoStephen C Jameson
Oct 31, 2018·Frontiers in Immunology·Elizabeth M KolawoleBrian D Evavold
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jenny S WongHsiu-Ching Chang
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lorna RettigRose Zamoyska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.