CD8+ T cell adjuvant effects of Salmonella FliCd flagellin in live vaccine vectors or as purified protein

Vaccine
Catarina J M BragaLuís C S Ferreira

Abstract

Salmonella flagellin, the flagellum structural subunit, has received particular interest as a vaccine adjuvant conferring enhanced immunogenity to soluble proteins or peptides, both for activation of antibody and cellular immune responses. In the present study, we evaluated the Salmonella enterica FliCd flagellin as a T cell vaccine adjuvant using as model the 9-mer (SYVPSAEQI) synthetic H2(d)-restricted CD8(+) T cell-specific epitope (CS(280-288)) derived from the Plasmodium yoelii circumsporozoite (CS) protein. The FliCd adjuvant effects were determined under two different conditions: (i) as recombinant flagella, expressed by orally delivered live S. Dublin vaccine strains expressing the target CS(280-288) peptide fused at the central hypervariable domain, and (ii) as purified protein in acellular vaccines in which flagellin was administered to mice either as a recombinant protein fused or admixed with the target CS(280-288) peptide. The results showed that CS(280-288)-specific cytotoxic CD8(+) T cells were primed when BALB/c mice were orally inoculated with the expressing the CS(280-288) epitope S. Dublin vaccine strain. In contrast, mice immunized with purified FliCd admixed with the CS(280-288) peptide and, to a lesser ext...Continue Reading

References

Mar 1, 1990·The Journal of Experimental Medicine·W R WeissS L Hoffman
Oct 1, 1990·The Journal of Experimental Medicine·A AggarwalJ Sadoff
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A MalikS L Hoffman
Feb 1, 1991·Molecular Microbiology·S M NewtonB A Stocker
Mar 6, 1998·Infection and Immunity·F Ciacci-WoolwineS B Mizel
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T D Eaves-PylesR B Pyles
Apr 23, 2004·Infection and Immunity·Camilo CuadrosJoseph Lustgarten
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arnaud DidierlaurentJean-Claude Sirard
Aug 27, 2005·Immunologic Research·Anna N Honko, Steven B Mizel
Feb 24, 2006·Nature Reviews. Immunology·Adrian V S Hill
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine J SandersAndrew T Gewirtz
May 22, 2007·Trends in Parasitology·D Gray HeppnerAdrian J F Luty
Jan 20, 2009·European Journal of Immunology·Catherine J SandersAndrew T Gewirtz
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·John T BatesSteven B Mizel

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Citations

May 24, 2011·Journal of Parasitology Research·Daniel Y BargieriMauricio M Rodrigues
May 28, 2013·BioMed Research International·Elena MataJosé Luis Pedraz
Jul 31, 2012·Therapeutic Delivery·Yoshio Fujita, Hiroaki Taguchi
Jan 15, 2013·ChemMedChem·Peter Michael Moyle, Istvan Toth
Nov 27, 2014·Human Vaccines & Immunotherapeutics·Mevyn NizardEric Tartour
Sep 7, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Christopher P KarchPeter Burkhard
Mar 28, 2018·Expert Review of Vaccines·Baofeng CuiYonglu Wang
Sep 30, 2016·World Journal of Methodology·Mozhgan Derakhshan SefidiShahram Nazarian
Nov 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steven B Mizel, John T Bates
Apr 26, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·John T BatesSteven B Mizel
Sep 2, 2017·Experimental & Molecular Medicine·Irshad A HajamJohn Hwa Lee
Oct 26, 2016·Biochemical Pharmacology·Christopher P Karch, Peter Burkhard

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