Abstract
CD3+CD8+ cells, purified from peripheral blood T cells by depletion of CD4+ lymphocytes, were tested for their cytotoxic activity against K-562 or HL-60 cells. Freshly prepared cells had no cytotoxic function, but upon exposure to recombinant interleukin 2 (rIL2) in vitro they acquired a lymphokine-activated killer (LAK) activity. Fractionation of CD3+CD8+ cells into CD11b+ and CD11b- cells demonstrated that all the cells with the potential of developing LAK cell functions were within the CD8+CD11b+ subset. These cells lacked natural killer cell markers such as CD16 or NKH1, but a proportion of them stained for Leu-7. Furthermore, they expressed the alpha/beta chains, but not the gamma/delta chains, of the T cell receptor, as could be determined by staining with the appropriate monoclonal antibodies. CD8+CD11b+ cells had a large granular lymphocyte morphology, as shown by both cytochemical and electron microscopy analyses. They proliferated in response to IL2 in vitro and developed cytotoxic functions against a number of natural killer resistant targets. Their response to phytohemagglutinin or pokeweed mitogen was very weak or absent. By contrast, CD8+CD11b- cells failed to generate LAK cells in response to rIL2, did not show a...Continue Reading
References
Dec 3, 1987·Nature·C MorimotoS F Schlossman
Aug 1, 1987·The Journal of Experimental Medicine·F CozzolinoM Ferrarini
Feb 1, 1988·Cellular Immunology·T TakeuchiC Morimoto
Apr 1, 1988·The Journal of Experimental Medicine·E CicconeL Moretta
Nov 1, 1986·Journal of Clinical Immunology·V PistoiaC E Grossi
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R J RobbW C Greene
Nov 14, 1986·Science·M SharonW J Leonard
Jan 15, 1987·International Journal of Cancer. Journal International Du Cancer·S FerriniA Moretta
Jan 1, 1987·The Journal of Experimental Medicine·K TeshigawaraK A Smith
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TsudoT A Waldmann
Feb 19, 1987·Nature·J BorstR L Bolhuis
Feb 19, 1987·Nature·M B BrennerM S Krangel
Feb 19, 1987·Nature·P MoingeonT Hercend
Jul 1, 1987·The Journal of Experimental Medicine·S FerriniA Moretta
Aug 1, 1987·The Journal of Experimental Medicine·E J FoxR R Rich
Sep 1, 1986·The Journal of Experimental Medicine·J H Phillips, L L Lanier
Oct 30, 1987·Science·H BandM B Brenner
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Feb 1, 1981·The Journal of Experimental Medicine·J A LedbetterL A Herzenberg
Jun 1, 1982·The Journal of Experimental Medicine·E A GrimmS A Rosenberg
Jul 1, 1983·The Journal of Experimental Medicine·N K Damle, E G Engleman
Nov 1, 1984·British Journal of Haematology·A C NewlandS Varadi
Oct 1, 1984·The Journal of Experimental Medicine·G TrinchieriB Perussia
Jul 1, 1980·European Journal of Immunology·E L ReinherzS F Schlossman
Sep 1, 1984·Immunology Today·I Lefkovits, H Waldmann
Citations
Apr 1, 1996·Cancer·J BrittendenO Eremin
Jan 1, 1994·Rheumatology International·G VaiopoulosG D Eliopoulos
Jul 1, 1992·Journal of Clinical Immunology·K YonedaT Osaki
May 1, 1993·Journal of Clinical Immunology·S ZupoM Ferrarini
Jul 1, 1993·Cancer Immunology, Immunotherapy : CII·I PrigioneV Pistoia
Aug 1, 1990·Mechanisms of Ageing and Development·L J Jenski
Mar 16, 1991·Lancet·K G ParkO Eremin
Feb 1, 1991·Current Opinion in Immunology·B Perussia
Jun 25, 1998·Developmental and Comparative Immunology·T L HortonJ D Horton
Jun 6, 1998·Experimental Gerontology·N BoucherF Schächter
Aug 29, 2002·Clinical and Experimental Immunology·R GerliC Lunardi
Mar 1, 2002·Immunology and Cell Biology·Fernando A Arosa
Jan 18, 2002·Infection and Immunity·Christopher B Forsyth, Herbert L Mathews
Jan 1, 1994·Leukemia & Lymphoma·M J Robertson, J Ritz
Aug 1, 1997·The Journal of Laboratory and Clinical Medicine·F W BeckA S Prasad
Oct 1, 1995·Clinical Immunology and Immunopathology·F Komatsu, K Kihara
Aug 1, 1991·Clinical Immunology and Immunopathology·M S FreedmanJ P Antel
Apr 1, 1990·Immunological Reviews·R S Larson, T A Springer
Jul 1, 1993·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·T YamamotoE Ueta
Dec 12, 1997·Alcoholism, Clinical and Experimental Research·E J LivantS J Ewald
Jan 1, 1990·Clinical and Experimental Immunology·M MassaiaA Pileri
Feb 11, 1992·European Journal of Immunology·U DianzaniK Bottomly
Jan 1, 1989·European Journal of Haematology. Supplementum·A PileriM Boccadoro
Oct 13, 2020·Frontiers in Immunology·Sara BruzzanitiMario Galgani