CD9, a key actor in the dissemination of lymphoblastic leukemia, modulating CXCR4-mediated migration via RAC1 signaling.

Blood
Marie-Pierre ArnaudVirginie Gandemer

Abstract

CD9, a member of the tetraspanin family, has been implicated in hematopoietic and leukemic stem cell homing. We investigated the role of CD9 in the dissemination of B acute lymphoblastic leukemia (B-ALL) cells, by stably downregulating CD9 in REH and NALM6 cells. CD9 expression was associated with higher levels of REH cell adhesion to fibronectin and C-X-C motif chemokine receptor 4 (CXCR4)-mediated migration. Death occurred later in NOD/SCID mice receiving REH cells depleted of CD9 for transplantation than in mice receiving control cells. After C-X-C motif chemokine ligand 12 (CXCL12) stimulation, CD9 promoted the formation of long cytoplasmic actin-rich protrusions. We demonstrated that CD9 enhanced RAC1 activation, in both REH cells and blasts from patients. Conversely, the overexpression of a competing CD9 C-terminal tail peptide in REH cytoplasm decreased RAC1 activation and cytoplasmic extension formation in response to CXCL12. Finally, the inhibition of RAC1 activation decreased migration in vitro, and the depletion of RAC1 protein from transplanted REH cells increased mouse survival. Furthermore, a testis-conditioned medium induced the migration of REH and NALM6 cells, and this migration was impeded by an anti-CD9 antib...Continue Reading

References

Dec 1, 1994·European Journal of Immunology·E RubinsteinC Boucheix
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·T NagasawaT Kishimoto
Sep 1, 1996·The Journal of Experimental Medicine·C C BleulT A Springer
Oct 2, 2001·Cellular and Molecular Life Sciences : CMLS·C Boucheix, E Rubinstein
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Jul 19, 2003·Scandinavian Journal of Immunology·E Severinson, L Westerberg
Sep 5, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Jul 19, 2005·Nature Medicine·Jose A CancelasDavid A Williams
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Martin E Hemler
Apr 5, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jia-Chi WangMario Chevrette
Feb 6, 2008·Journal of Cellular Physiology·Yoko MurayamaNorio Hayashi
Dec 17, 2008·Nature Reviews. Cancer·Margot Zöller
Mar 4, 2009·Biochemical and Biophysical Research Communications·Hiroko NishidaChikao Morimoto
Jul 1, 2009·Biochemical and Biophysical Research Communications·Jiying WangJianxiang Wang
Jul 20, 2010·Seminars in Cancer Biology·Sigal Tavor, Isabelle Petit
Jan 12, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ching-Hon PuiRobert J Arceci
May 10, 2011·Biochemical and Biophysical Research Communications·Hiroto YamazakiChikao Morimoto
Jul 21, 2011·Journal of Cell Science·Hong-Xing WangMartin E Hemler
Oct 9, 2012·Cell Stem Cell·Mito Kanatsu-ShinoharaTakashi Shinohara

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Citations

Jan 14, 2017·ELife·Agnė Kozlovskaja-GumbrienėTatjana Piotrowski
Dec 30, 2017·Cancer Biomarkers : Section a of Disease Markers·Peiqi LiangRong Hu
May 3, 2018·Journal of Leukocyte Biology·Laura Martínez-MuñozMario Mellado
Sep 29, 2018·Cancers·Laurie G HudsonAngela Wandinger-Ness
Aug 23, 2018·Molecular Biology of the Cell·Chelsea A Saito-ReisJennifer M Gillette
Jul 29, 2016·World Journal of Gastroenterology : WJG·Sarah HeilerMargot Zöller
Apr 22, 2017·Frontiers in Cell and Developmental Biology·Christina M Termini, Jennifer M Gillette
Dec 19, 2018·International Journal of Molecular Sciences·Valerie Durand-OnaylıTanja Nicole Hartmann
Feb 23, 2019·Frontiers in Immunology·Javier Redondo-MuñozJoaquin Teixidó
Dec 12, 2018·British Journal of Haematology·Ma Reina ImprogoJennifer R Brown
Jul 28, 2019·Journal of Immunology Research·Georgia TsaouliMaria Pia Felli
Dec 14, 2018·Journal of Experimental & Clinical Cancer Research : CR·Kun ZhaoMargot Zöller
Mar 22, 2021·Journal of Hematology & Oncology·Zixi HongLiang Shao
Jun 12, 2021·Biomarker Research·Xuanmei HuangHua Zhang

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