CD90 Identifies Adventitial Mesenchymal Progenitor Cells in Adult Human Medium- and Large-Sized Arteries

Stem Cell Reports
Katherine C MichelisJason C Kovacic

Abstract

Mesenchymal stem cells (MSCs) reportedly exist in a vascular niche occupying the outer adventitial layer. However, these cells have not been well characterized in vivo in medium- and large-sized arteries in humans, and their potential pathological role is unknown. To address this, healthy and diseased arterial tissues were obtained as surplus surgical specimens and freshly processed. We identified that CD90 marks a rare adventitial population that co-expresses MSC markers including PDGFRα, CD44, CD73, and CD105. However, unlike CD90, these additional markers were widely expressed by other cells. Human adventitial CD90+ cells fulfilled standard MSC criteria, including plastic adherence, spindle morphology, passage ability, colony formation, and differentiation into adipocytes, osteoblasts, and chondrocytes. Phenotypic and transcriptomic profiling, as well as adoptive transfer experiments, revealed a potential role in vascular disease pathogenesis, with the transcriptomic disease signature of these cells being represented in an aortic regulatory gene network that is operative in atherosclerosis.

Citations

May 2, 2020·Journal of the American College of Cardiology·Javier Barallobre-BarreiroJason C Kovacic
Dec 24, 2019·The American Journal of Pathology·Maria G Tinajero, Avrum I Gotlieb
Mar 20, 2021·Cardiovascular Research·Alexandre PersuAndrzej Januszewicz
Dec 21, 2021·Frontiers in Cardiovascular Medicine·Severi MulariEsko Kankuri

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Methods Mentioned

BETA
flow cytometry
Fluorescence-activated cell sorting
RNA-seq
coronary artery bypass

Software Mentioned

STARNET RGN
STARNET

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