CD95 (Fas/APO-1) antibody-mediated apoptosis of human retinal pigment epithelial cells

Biochemical and Biophysical Research Communications
P EsserM Weller

Abstract

CD95 (Fas/APO-1) is a cytokine receptor protein that signals apoptosis. Here we report that human retinal pigment epithelial cells express CD95 but are rather resistant to agonistic CD95 antibodies. Resistance to CD95 antibodies is overcome by preexposure to the cytokines, tumor necrosis factor-alpha or interferon-gamma, or by coexposure to CD95 antibodies and inhibitors of RNA or protein synthesis. The cells are resistant to tumor necrosis factor-alpha even in the presence of cycloheximide and only moderately sensitized to tumor necrosis factor-alpha-mediated toxicity by coexposure to actinomycin D. CD95-mediated apoptosis is inhibited by dexamethasone both after cytokine sensitization and upon coexposure to CD95 antibodies and actinomycin D or cycloheximide. Induction of apoptosis via CD95 may be involved in the regulation of retinal pigment epithelial proliferation at the vitreoretinal interface.

Citations

Sep 12, 2002·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Yu-Sheng WangPeter Wiedemann
Apr 25, 2000·The Tohoku Journal of Experimental Medicine·H TomitaM Tamai
Jan 8, 2000·Fundamental & Clinical Pharmacology·A TrifilieffC Bertrand
Dec 31, 1997·The American Journal of Physiology·L P WenG D Rosen
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Jul 26, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Christin HettichAlexa Klettner
Jul 19, 2002·Experimental Eye Research·Arno HueberPeter Johannes Esser
May 10, 2006·Experimental Eye Research·Deborah A FerringtonDale S Gregerson
Sep 4, 2021·Ocular Immunology and Inflammation·Henry J KaplanHui Shao

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