Cdc13 and telomerase bind through different mechanisms at the lagging- and leading-strand telomeres

Molecular Cell
Virginie FaureVincent Géli

Abstract

Lagging-strand and leading-strand synthesis of chromosomes generates two structurally distinct ends at the telomeres. Based on sequence bias of yeast telomeres that contain a 250-300 bp array of C(1-3)A/ TG(1-3) repeats, we developed a method allowing us to distinguish which of the two daughter telomeres chromosome end-binding proteins bind to at the end of S phase. The single-stranded DNA-binding protein Cdc13 and the telomerase subunits Est1 and Est2 can bind to the two daughter telomeres, but only their binding to the leading-strand telomere depends on the Mre11/Rad50/Xrs2 (MRX) complex involved in both telomeric 5' nucleolytic resection and telomerase recruitment at short telomeres. Consistently, the MRX complex is mainly found to bind to the leading-strand telomere. Our results indicate that Cdc13 can bind to the telomeric template for lagging-strand replication. Since mre11-deficient strains have markedly short telomeres, telomere elongation by telomerase is likely to occur mainly at the leading-strand telomere.

References

Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J Lin, V A Zakian
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·I Dionne, R J Wellinger
Nov 25, 1998·Nucleic Acids Research·I Dionne, R J Wellinger
Jun 16, 1999·The EMBO Journal·S MarcandE Gilson
Oct 3, 1999·Science·S K Evans, V Lundblad
Jan 11, 2000·Molecular and Cellular Biology·A Adams MartinC Holm
May 10, 2000·Current Biology : CB·S MarcandE Gilson
Mar 7, 2001·Genes & Development·A ChandraV Lundblad
Aug 4, 2001·Molecular and Cellular Biology·R OhkiF Ishikawa
Sep 29, 2001·Science·S M BaileyE H Goodwin
May 25, 2002·Molecular and Cellular Biology·Wonchae ChoeJudith L Campbell
Aug 10, 2002·Science·Andrew K P TaggartVirginia A Zakian
Jan 1, 2004·Nature Genetics·Vera SchramkeVincent Géli
Jun 17, 2004·Genes & Development·Michel LarrivéeRaymund J Wellinger
Nov 9, 2004·Nature Structural & Molecular Biology·Timothy S FisherVirginia A Zakian
Feb 4, 2006·Molecular Cell·Weihang ChaiWoodring E Wright
Oct 5, 2006·Molecular Cell·Momchil D Vodenicharov, Raymund J Wellinger
Oct 31, 2006·Molecular Cell·Christopher J FrankCarol W Greider
Feb 13, 2007·Nature Structural & Molecular Biology·Hua GaoVictoria Lundblad
Apr 13, 2007·Molecular Biology of the Cell·Tatsuya KibeMasaru Ueno
Jul 20, 2007·Genes & Development·Alessandro Bianchi, David Shore
Jul 28, 2007·Molecular Cell·Michelle SabourinVirginia A Zakian
Sep 7, 2007·Molecular Cell·Ronald E HectorKurt W Runge
Sep 22, 2007·Nature Reviews. Molecular Cell Biology·Eric Gilson, Vincent Géli
Mar 6, 2008·Molecular Systems Biology·Rafi ShacharRoded Sharan
Jul 29, 2008·Molecular Cell·Alessandro Bianchi, David Shore

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Citations

Jun 19, 2012·Nature Structural & Molecular Biology·Christian B SchillerKarl-Peter Hopfner
Jul 20, 2012·Genes & Development·Anita KazdaKarel Riha
Oct 15, 2013·Chromosoma·Diego BonettiMaria Pia Longhese
Jul 22, 2010·The EMBO Journal·Maria Pia LongheseMichela Clerici
Apr 23, 2014·FEMS Microbiology Reviews·Martin Kupiec
May 28, 2013·Cell Cycle·Magda GranataMarco Muzi-Falconi
Mar 25, 2014·Molecular Cell·Julien SoudetMaria Teresa Teixeira
Sep 14, 2010·Molecular Cell·Marie-Josèphe Giraud-PanisEric Gilson
Jan 15, 2016·Cold Spring Harbor Symposia on Quantitative Biology·Kah Wai Lin, Virginia A Zakian
Feb 23, 2012·The EMBO Journal·Pierre LucianoVincent Géli
Jul 1, 2014·Molecular & Cellular Oncology·Stéphane Marcand
Jul 13, 2016·Genes & Development·Carol W Greider
Aug 22, 2013·The Journal of Biological Chemistry·Martin E Budd, Judith L Campbell
Feb 6, 2017·Nucleic Acids Research·Daniele CesenaMaria Pia Longhese
Jan 9, 2020·Frontiers in Cell and Developmental Biology·Evelyne Ségal-Bendirdjian, Vincent Geli
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Jee Whu Lee, Eugene Boon Beng Ong
Mar 27, 2021·Biochemical Society Transactions·Michela GalliMichela Clerici
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Erin BonnellRaymund J Wellinger
Jun 24, 2021·Nature Communications·Freddy KhayatAlessandro Bianchi

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