Cdc14b regulates mammalian RNA polymerase II and represses cell cycle transcription.

Scientific Reports
María GuillamotMarcos Malumbres

Abstract

Cdc14 is an essential phosphatase in yeast but its role in the mammalian cell cycle remains obscure. We report here that Cdc14b-knockout cells display unscheduled induction of multiple cell cycle regulators resulting in early entry into DNA replication and mitosis from quiescence. Cdc14b dephosphorylates Ser5 at the C-terminal domain (CTD) of RNA polymerase II, a major substrate of cyclin-dependent kinases. Lack of Cdc14b results in increased CTD-Ser5 phosphorylation, epigenetic modifications that mark active chromatin, and transcriptional induction of cell cycle regulators. These data suggest a function for mammalian Cdc14 phosphatases in the control of transcription during the cell cycle.

References

Oct 13, 2001·The Journal of Biological Chemistry·J Bembenek, H Yu
Mar 21, 2002·Nature Reviews. Cancer·M Malumbres, M Barbacid
Aug 28, 2003·Nature Structural Biology·Stephen Buratowski
Sep 27, 2003·European Journal of Biochemistry·Benoît Palancade, Olivier Bensaude
Dec 1, 2004·Annual Review of Genetics·Frank Stegmeier, Angelika Amon
May 25, 2005·The Journal of Biological Chemistry·M Dolores Vázquez-NovelleMaría P Sacristán
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Oct 21, 2005·Trends in Biochemical Sciences·Marcos Malumbres, Mariano Barbacid
May 7, 2008·Trends in Genetics : TIG·Sylvain Egloff, Shona Murphy
Jun 17, 2008·Nature Cell Biology·Irene García-HigueraMarcos Malumbres
Jul 30, 2008·Cell·Florian BassermannMichele Pagano
Oct 11, 2008·Current Opinion in Cell Biology·Ethel Queralt, Frank Uhlmann
Oct 14, 2009·Molecular and Cellular Biology·Daniel S GinsburgAlan G Hinnebusch
Nov 28, 2009·Molecular Cell·Stephen Buratowski
Aug 20, 2010·Journal of Cell Science·Annamaria Mocciaro, Elmar Schiebel
Oct 20, 2010·The Journal of Biological Chemistry·María D Vázquez-NovelleMaría P Sacristán
Jan 26, 2011·Molecular and Cellular Biology·Zhubo WeiPumin Zhang

❮ Previous
Next ❯

Citations

Dec 12, 2012·Nature Biotechnology·Cole TrapnellLior Pachter
Oct 12, 2012·Molecular Biology of the Cell·Sara OvejeroMaría P Sacristán
Aug 25, 2012·Molecular Biology of the Cell·Matthew R Broadus, Kathleen L Gould
Jan 7, 2014·PLoS Genetics·Nadia HégaratHelfrid Hochegger
Jan 19, 2016·Biochimica Et Biophysica Acta·Joshua E MayfieldYan Jessie Zhang
Jun 4, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mónica Alvarez-Fernández, Marcos Malumbres
Aug 19, 2015·Nature Biotechnology·Alan S L WongTimothy K Lu
Aug 5, 2015·Biotechnology Advances·Rakesh Srivastava, Seong Hoon Ahn
Sep 18, 2012·Biochimica Et Biophysica Acta·Martin HeidemannDirk Eick
May 28, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nadia HégaratHelfrid Hochegger
Jan 9, 2013·Molecular & Cellular Proteomics : MCP·Jun-Song ChenKathleen L Gould
Jun 28, 2018·Biology Open·Leif R NeitzelEthan Lee
Nov 18, 2016·The EMBO Journal·María Teresa VilloriaAndrés Clemente-Blanco
Jan 25, 2020·International Journal of Molecular Sciences·Javier Manzano-López, Fernando Monje-Casas
Mar 19, 2020·International Journal of Molecular Sciences·Lei DingQinghua Cui
Jan 16, 2020·International Journal of Molecular Sciences·Adrián Campos, Andrés Clemente-Blanco
Dec 23, 2015·BMC Genomics·Borhan UddinElmar Schiebel
Jul 11, 2013·Chemical Reviews·Célia JeronimoFrançois Robert

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
pull down
acetylation
ubiquitination
glycosylation
genotyping
electrophoresis

Software Mentioned

Bioconductor
limma
TM4

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.