Cdc25C expression in meiotically competent and incompetent goat oocytes

Molecular Reproduction and Development
L GallClaire Boulesteix

Abstract

Change in Cdc25C expression and localization during maturation and meiotic competence acquisition was investigated in goat oocytes. Western blot analysis revealed that Cdc25C is constitutively expressed throughout meiosis in competent goat oocytes, with changes in its phosphorylation level. Cdc25C was detected at 55 and 70 kDa, representing the nonphosphorylated form and the hyperphosphorylated active form, respectively. During the G2-M transition at meiosis resumption, Cdc25C was hyperphosphorylated as evidenced by a clear shift from 55 to 70 kDa. Okadaic acid which induced premature meiosis resumption associated with MPF activation also involved a premature shift from 55 to 70 kDa in goat competent oocytes. After artificial activation of goat oocytes, Cdc25C returned to its 55 kDa form. By indirect immunofluorescence, Cdc25C was found essentially localized in the nucleus at the germinal vesicle stage, suggesting that Cdc25C functions within the nucleus to regulate MPF activation. Concomitantly with germinal vesicle breakdown, Cdc25C was redistributed throughout the cytoplasm. The amount of Cdc25C, very low in incompetent oocytes, increased with meiosis competence acquisition. On the other hand, during oocyte growth while the ...Continue Reading

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Citations

Mar 31, 2006·Human Reproduction·Said AssouSamir Hamamah
Feb 26, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Laurence GallXavier Vignon
Mar 3, 2004·Molecular Reproduction and Development·Laurence GallClaire Boulesteix
Dec 1, 2004·Molecular Reproduction and Development·Cean LaRosa, Stephen M Downs

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