CDC36 and CDC39 are negative elements in the signal transduction pathway of yeast

Cell Regulation
A M NeimanI Herskowitz

Abstract

Mutations in either the CDC36 or CDC39 gene cause yeast cells to arrest in G1 of the cell cycle at the same point as treatment with mating pheromone. We demonstrate here that strains harboring temperature-sensitive mutations in CDC36 or CDC39 activate expression of the pheromone-inducible gene FUS1 when shifted to nonpermissive temperature. We show further that cell-cycle arrest and induction of FUS1 are dependent on known components of the mating factor response pathway, the STE genes. Thus, the G1-arrest phenotype of cdc36 and cdc39 mutants results from activation of the mating factor response pathway. The CDC36 and CDC39 gene products behave formally as negative elements in the response pathway: they are required to block response in the absence of pheromone. Epistasis analysis of mutants defective in CDC36 or CDC39 and different STE genes demonstrates that activation requires the response pathway G protein and suggests that CDC36 and CDC39 products may control synthesis or function of the G alpha subunit.

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A HinnenG R Fink
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J W DolanS Fields
Jan 1, 1988·Annual Review of Cell Biology·F CrossJ B Konopka
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·S I ReedK Y Jahng
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D C HagenG F Sprague
Jan 1, 1986·Annual Review of Cell Biology·L Stryer, H R Bourne
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
May 12, 1988·Nature·E J Neer, D E Clapham
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M A TeagueB Errede
Jan 11, 1974·Science·L H HartwellB J Reid
Oct 5, 1984·Journal of Molecular Biology·M SternI Herskowitz

❮ Previous
Next ❯

Citations

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C OppiG P Tocchini Valentini
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·M R GerberD O Morgan
Apr 28, 2011·The Journal of Biological Chemistry·Matthew P TorresHenrik G Dohlman
Oct 11, 1991·Cell Regulation·K L ClarkG F Sprague
Aug 1, 1992·Antonie van Leeuwenhoek·J B Konopka, S Fields
Oct 1, 1991·Current Opinion in Genetics & Development·S I Reed
May 16, 1991·Nature·H R Bourne
May 15, 2010·Expert Opinion on Investigational Drugs·Alexander Schultze, Walter Fiedler
Nov 4, 2020·Cells·Nafiseh Chalabi Hagkarim, Roger J Grand
Feb 16, 1991·Biochimica Et Biophysica Acta·J W Dolan, S Fields
Sep 1, 1992·Trends in Genetics : TIG·L Avery, S Wasserman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.