CDC42 Gtpase Activation Affects Hela Cell DNA Repair and Proliferation Following UV Radiation-Induced Genotoxic Stress

Journal of Cellular Biochemistry
Liv G AscerFabio Luis Forti

Abstract

Cell division control protein 42 (CDC42) homolog is a small Rho GTPase enzyme that participates in such processes as cell cycle progression, migration, polarity, adhesion, and transcription. Recent studies suggest that CDC42 is a potent tumor suppressor in different tissues and is related to aging processes. Although DNA damage is crucial in aging, a potential role for CDC42 in genotoxic stress remains to be explored. Migration, survival/proliferation and DNA damage/repair experiments were performed to demonstrate CDC42 involvement in the recovery of HeLa cells exposed to ultraviolet radiation-induced stress. Sub-lines of HeLa cells ectopically expressing the constitutively active CDC42-V12 mutant were generated to examine whether different CDC42-GTP backgrounds might reflect different sensitivities to UV radiation. Our results show that CDC42 constitutive activation does not interfere with HeLa cell migration after UV radiation. However, the minor DNA damage exhibited by the CDC42-V12 mutant exposed to UV radiation most likely results in cell cycle arrest at the G2/M checkpoint and reduced proliferation and survival. HeLa cells and Mock clones, which express endogenous wild-type CDC42 and show normal activity, are more resista...Continue Reading

References

Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·D I Johnson
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Nov 8, 2001·Current Opinion in Cell Biology·J Bartek, J Lukas
May 15, 2002·The EMBO Journal·Gilles GadéaPierre Roux
Aug 15, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D F R MurisR J A M Michalides
Feb 19, 2004·The Journal of Biological Chemistry·MiRan SeoYong-Sung Juhnn
Mar 9, 2004·Trends in Cell Biology·Richard A Cerione
Jul 21, 2004·The Journal of Biological Chemistry·Kyung A ChoSang Chul Park
Jul 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Takao KamaiKen-Ichiro Yoshida
Feb 14, 2006·Methods in Enzymology·Qiong LinRichard A Cerione
May 24, 2006·FEBS Letters·Shingo YasudaShuh Narumiya
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lei WangYi Zheng
Oct 6, 2007·Molecular Neurobiology·Evgeni PonimaskinAlexander Dityatev
Aug 23, 2008·Nature Reviews. Molecular Cell Biology·Sarah J Heasman, Anne J Ridley
Oct 7, 2008·Current Biology : CB·Gilles GadeaChristopher J Marshall
Feb 28, 2009·Aging Cell·Richard A KerberRichard M Cawthon
Dec 1, 2010·The Journal of Biological Chemistry·Jaime MelendezYi Zheng
Dec 7, 2010·DNA Repair·Zoraya Palomera-Sanchez, Mario Zurita
Apr 26, 2011·Cellular Signalling·Kristy Stengel, Yi Zheng
May 24, 2011·Mutation Research·Alex A Freitas, João Pedro de Magalhães
May 31, 2011·Frontiers in Bioscience (Landmark Edition)·Rajamani RathinamSuresh K Alahari
Sep 8, 2011·Protein Science : a Publication of the Protein Society·Wei Yang
Sep 29, 2011·British Journal of Cancer·R DeeviF C Campbell
Jan 24, 2012·International Journal of Oncology·Qing-Yong ChenJian-Ying Zhou
May 9, 2012·Cell Stem Cell·Maria Carolina FlorianHartmut Geiger
Jan 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Amy FrieslandQun Lu
Feb 6, 2013·The Journal of Biological Chemistry·Lin HongAngela Wandinger-Ness

❮ Previous
Next ❯

Citations

Jan 18, 2019·Journal of Cellular Physiology·Natsumi DoiMasaaki Tatsuka
Jul 26, 2018·Scientific Reports·Karina Griesi-OliveiraMaria Rita Passos-Bueno
Jan 26, 2020·Cancer Letters·Chun-Wen ZhengEn-Min Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.