CDC6 requirement for spindle formation during maturation of mouse oocytes

Biology of Reproduction
Martin AngerR M Schultz

Abstract

A master regulator of DNA replication, CDC6 also functions in the DNA-replication checkpoint by preventing DNA rereplication. Cyclin-dependent kinases (CDKs) regulate the amount and localization of CDC6 throughout the cell cycle; CDC6 phosphorylation after DNA replication initiation leads to its proteolysis in yeast or translocation to the cytoplasm in mammals. Overexpression of CDC6 during the late S phase prevents entry into the M phase by activating CHEK1 kinase that then inactivates CDK1/cyclin B, which is essential for the G2/M-phase transition. We analyzed the role of CDC6 during resumption of meiosis in mouse oocytes, which are arrested in the first meiotic prophase with low CDK1/cyclin B activity; this is similar to somatic cells at the G2/M-phase border. Overexpression of CDC6 in mouse oocytes does not prevent resumption of meiosis. The RNA interference-mediated knockdown of CDC6, however, reveals a new and unexpected function for CDC6; namely, it is essential for spindle formation in mouse oocytes.

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Citations

Jul 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karen SchindlerRichard M Schultz
Jan 23, 2009·The Journal of Biological Chemistry·Aïcha MetchatElisabeth S Christians
Mar 21, 2007·Genes & Development·Elizabeth P MurchisonGregory J Hannon
Jun 27, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Tereza ToralováJiří Kaňka
Aug 5, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Tereza ToralováJirí Kanka
Sep 30, 2014·Developmental Biology·Mohammed El DikaJacek Z Kubiak
May 23, 2015·Molecular Human Reproduction·Jessica E FellmethKaren Schindler
Jan 21, 2012·Cell Cycle·Yadushyla NarasimhacharMartine Coué
Mar 18, 2008·Developmental Biology·Hua PanRichard M Schultz
Jun 25, 2005·Developmental Biology·Fanyi Zeng, Richard M Schultz
Sep 30, 2005·Cytogenetic and Genome Research·U Eichenlaub-Ritter
Jan 15, 2016·Molecular Biology of the Cell·Xue-Shan MaQing-Yuan Sun
Apr 4, 2017·Molecular Human Reproduction·Alexandra L NguyenKaren Schindler
Feb 21, 2018·Biology of Reproduction·Richard M SchultzPetr Svoboda
Oct 7, 2017·Human Molecular Genetics·Likun YaoAnming Meng
Aug 11, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eric LauWei Jiang
Feb 16, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hyun-Seo LeeKyung-Ah Lee
Apr 24, 2016·Human Molecular Genetics·Jessica E FellmethKaren Schindler
Jul 21, 2006·Biology of Reproduction·Ludmila G RomanovaRichard M Schultz

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Methods Mentioned

BETA
in vitro transcription
PCR

Software Mentioned

ImageQuant
Prism Pad

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