CDH13 abundance interferes with adipocyte differentiation and is a novel biomarker for adipose tissue health

International Journal of Obesity : Journal of the International Association for the Study of Obesity
Simon GöddekeJorg Kotzka

Abstract

CDH13, an atypical member of the cadherin superfamily, has been identified in adipocyte secretomes of lean mouse models. CDH13 abundance differs in mouse models according to their susceptibility to develop metabolic disorders, but the role of CDH13 in adipose tissue is unknown. Secreted CDH13 protein levels and mRNA levels in visceral adipose tissue were determined in lean and obese mouse models. In vitro studies were performed in 3T3-L1 adipocytes to determine the role of CDH13 in adipocyte differentiation. The pathophysiological impact of visceral adipose tissue CDH13 mRNA and circulating CDH13 levels were determined in humans (normal-weight men n = 37, obese men n = 109 including n = 51 type 2 diabetes patients) and in obese patients (n = 14) pre- and post-metabolic surgery. This study shows that in visceral adipose tissue CDH13 protein secretion and mRNA levels were decreased in obese mouse models. Mechanistically, CDH13 affects lipid metabolism during adipogenesis but not in mature adipocytes. CDH13 knockdown during adipogenesis reduced fatty acid uptake and lipid content in developing adipocytes. Furthermore, CDH13 depletion during adipogenesis lowered the induction of PPARγ and C/EBPα expression. These observations are o...Continue Reading

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Citations

Feb 5, 2021·The Journal of Clinical Endocrinology and Metabolism·Shiro FukudaIichiro Shimomura

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Methods Mentioned

BETA
gastric
gastric bypass
biopsies
FCS
PCRs
Assay
transfection

Clinical Trials Mentioned

NCT00740194

Software Mentioned

GraphPad Prism
QbasePlus
Proteome Discoverer
SPSS

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