CDK activity provides temporal and quantitative cues for organizing genome duplication

PLoS Genetics
Anthony PerrotPei-Yun Jenny Wu

Abstract

In eukaryotes, the spatial and temporal organization of genome duplication gives rise to distinctive profiles of replication origin usage along the chromosomes. While it has become increasingly clear that these programs are important for cellular physiology, the mechanisms by which they are determined and modulated remain elusive. Replication initiation requires the function of cyclin-dependent kinases (CDKs), which associate with various cyclin partners to drive cell proliferation. Surprisingly, although we possess detailed knowledge of the CDK regulators and targets that are crucial for origin activation, little is known about whether CDKs play a critical role in establishing the genome-wide pattern of origin selection. We have addressed this question in the fission yeast, taking advantage of a simplified cell cycle network in which cell proliferation is driven by a single cyclin-CDK module. This system allows us to precisely control CDK activity in vivo using chemical genetics. First, in contrast to previous reports, our results clearly show that distinct cyclin-CDK pairs are not essential for regulating specific subsets of origins and for establishing a normal replication program. Importantly, we then demonstrate that the t...Continue Reading

References

Jan 1, 1992·Annual Review of Genetics·J Hayles, P Nurse
Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Sep 1, 1996·Trends in Genetics : TIG·B Stern, P Nurse
Jan 1, 1997·Annual Review of Cell and Developmental Biology·D O Morgan
Sep 12, 1998·Molecular Cell·A D DonaldsonW L Fangman
Aug 13, 1999·Molecular Cell·F R CrossM D Jacobson
Jun 16, 2004·The Journal of Cell Biology·Susanna Ekholm-ReedSteven I Reed
Aug 19, 2004·Cell·Katarzyna KozarPiotr Sicinski
Oct 28, 2005·Molecular Biology of the Cell·Prasanta K PatelNicholas Rhind
Feb 25, 2006·Yeast·Susan L Forsburg, Nicholas Rhind
Oct 21, 2006·The EMBO Journal·Christian HeichingerPaul Nurse
Aug 19, 2007·Nature·David SantamaríaMariano Barbacid
Oct 10, 2008·PLoS Biology·Ichiro HirataniDavid M Gilbert
Feb 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yuko KatsunoMakoto Nakanishi
Sep 22, 2009·Genome Research·Romain DespratEric E Bouhassira
Dec 17, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Benjamin D PopeDavid M Gilbert
Jan 20, 2010·The Journal of Cell Biology·Alexander M ThomsonJ Julian Blow
Dec 24, 2010·Nature·Damien Coudreuse, Paul Nurse
Sep 3, 2011·Science·Nagarajan Nandagopal, Michael B Elowitz
May 15, 2012·Methods : a Companion to Methods in Enzymology·Julien N BiancoPhilippe Pasero
Jul 19, 2013·Development·Shuhui Lim, Philipp Kaldis
Feb 25, 2014·Molecular Cell·Pei-Yun Jenny Wu, Paul Nurse
Feb 11, 2015·Nature Structural & Molecular Biology·Yasukazu DaigakuAntony M Carr
Sep 28, 2015·Trends in Cell Biology·Stephen Dalton
Jan 20, 2016·Scientific Reports·Atanas KaykovPaul Nurse
Dec 17, 2016·Cell·Matthew P SwafferPaul Nurse

❮ Previous
Next ❯

Citations

Jun 22, 2018·Current Genetics·Balveer Singh, Pei-Yun Jenny Wu
Jan 3, 2019·Current Genetics·Balveer Singh, Pei-Yun Jenny Wu
Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Julien MambuAgnès Wiedemann

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE88714

Methods Mentioned

BETA
flow cytometry
immunoprecipitation
immunoprecipitations
PCR
ChIP-chip
ChIP

Software Mentioned

VisiView
Flowjo

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Related Papers

WormBook : the Online Review of C. Elegans Biology
Sander van den Heuvel
Nature Reviews. Molecular Cell Biology
Joanna Bloom, Frederick R Cross
© 2022 Meta ULC. All rights reserved