PMID: 2116765Aug 15, 1990Paper

cDNA and deduced amino acid sequences of a dog hepatic cytochrome P450IIB responsible for the metabolism of 2,2',4,4',5,5'-hexachlorobiphenyl

Archives of Biochemistry and Biophysics
P E GravesJ R Halpert

Abstract

The nucleotide sequence of a cDNA that codes for the major phenobarbital (PB)-inducible male beagle dog hepatic cytochrome P450 has been determined. Using a rabbit P450IIB cDNA probe (R. Gasser, M. Negishi, and R. M. Philpot, 1988, Mol. Pharmacol, 32, 22-30), a cDNA clone with a 2.6-kilobase pair insert was isolated from a lambda gt11 library prepared from hepatic mRNA from a PB-treated dog. The cloned insert was sequenced and found to contain an open reading frame coding for a polypeptide of 494 amino acids (Mr 56,183). The encoded protein can be assigned to the P450IIB subfamily on the basis of homology to cytochromes P450 from other species. The deduced amino acid sequence is 79% identical to that reported for rabbit P450 BO (P450IIB4) and 75% identical to that for rat P450b (P450IIB1). The sequence identity decreases to less than 52% when the dog sequence is compared with other P450II subfamilies. The deduced NH2-terminal 30 amino acids encoded by the dog cDNA are identical to those determined by sequence analysis of purified dog cytochrome P450 PBD-2, and the amino acid composition concurs with that determined for the PBD-2 protein (D. B. Duignan, I. G. Sipes, T. B. Leonard, and J. R. Halpert, 1987, Arch. Biochem. Biophys....Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T KronbachE F Johnson
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P H BeauneF P Guengerich
Feb 1, 1987·DNA·D W NebertW Levin
Jan 29, 1986·Biochemical and Biophysical Research Communications·W PyerinH Taniguchi
Sep 14, 1968·Nature·H MetzgerJ E Vinton
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y MizukamiY Fujii-Kuriyama
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A Rampersaud, F G Walz
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K KawajiriY Fujii-Kuriyama
Feb 1, 1982·Toxicology and Applied Pharmacology·I G SipesD E Carter
Sep 15, 1982·Toxicology and Applied Pharmacology·I G SipesD E Carter
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Y Fujii-KuriyamaM Muramatsu

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Citations

Jun 28, 1994·Biochimica Et Biophysica Acta·P J Ciaccio, K D Tew
Feb 16, 1991·Biochimica Et Biophysica Acta·P J CiaccioJ R Halpert
Jul 10, 1993·Biochimica Et Biophysica Acta·K M KedzieJ R Halpert
Mar 1, 1995·The International Journal of Biochemistry & Cell Biology·Y Nishibe, M Hirata
Jun 29, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jean-Marie NicolasSophie Kervyn
Feb 26, 2013·Drug Metabolism Reviews·Marilyn N MartinezLauren Trepanier
Nov 28, 2006·Expert Opinion on Drug Metabolism & Toxicology·Marcella MartignoniRuben de Kanter
Jun 1, 1993·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Y Nishibe, M Hirata
Oct 5, 2010·Ecotoxicology and Environmental Safety·Maja KirkegaardPhilippe Grandjean
May 15, 1991·Archives of Biochemistry and Biophysics·K OguriH Yoshimura
Jan 25, 2000·The Journal of Biological Chemistry·M F OleksiakD C Zeldin
Jun 25, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Masashi MiseSetsuko Komuro
Jan 29, 2005·The Journal of Pharmacology and Experimental Therapeutics·Ping LuMagang Shou
Aug 16, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Magang ShouThomas H Rushmore
Oct 23, 1997·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·N AriyoshiK Oguri
Oct 19, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Richard A GrahamAndrew Parkinson
Jan 28, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Masashi MiseToshihiko Fujii
Mar 4, 2005·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D TenmizuH Kamimura
Mar 15, 2003·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M J GrahamM S Lennard
Nov 15, 1991·Archives of Biochemistry and Biophysics·K M KedzieJ R Halpert

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