cDNA-array profiling of melanomas and paired melanocyte cultures

Journal of Cellular Physiology
Carlo MischiatiPatrizio Giacomini

Abstract

Three paired (from the same donor) sets of melanoma cells and normal melanocytes, established as early-passage cultures from metastatic lesions and the uninvolved skin of three patients, were comparatively cDNA profiled by macroarrays (approximately 1,200 genes) and reverse transcription (RT)-PCR. While 145 gene products were significantly (at least twofold) upregulated or downregulated in at least 1 pair, and 23 were in at least 2 pairs, only 3 (the signal transducer and activator of transcription STAT2, collagen type VI, and CD9) were concordantly modulated (downregulation) in all 3 pairs. Array results were validated by RT-PCR on a small panel of surgically removed nevocellular nevi and metastatic melanoma lesions, and by immunohistochemistry on a large panel of benign and malignant lesions of the nevomelanocytic lineage. The three gene products were downregulated at different stages of melanoma progression. STAT2 was detectable in nevi (5/5) and most primary melanomas (11/12), but was lost in 10/15 metastatic lesions. Collagen type VI was expressed in nevi (5/5) and primary melanomas below a Breslow thickness of 1 mm (3/3), but was lost in 24/24 primary melanomas above this threshold, and in metastatic melanomas (10/10). Th...Continue Reading

References

Jan 1, 1990·Advances in Cancer Research·M HerlynU Rodeck
Apr 15, 1985·International Journal of Cancer. Journal International Du Cancer·P G NataliA Bigotti
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·S K LoftusW Pavan
Jun 13, 2000·Oncogene·T BowmanR Jove
Sep 25, 2001·The Journal of Pathology·A A AlizadehM van de Rijn
May 20, 2003·Pigment Cell Research·Thomas P DooleyTeresa W Wilborn
Jun 18, 2003·Journal of Cellular Physiology·Alfonso BaldiMarco G Paggi
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Pieter A van der VeldenNelleke A Gruis
Feb 23, 2005·Archives of Dermatology·Sandeep NambiarUlrich R Hengge

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Citations

May 24, 2008·Carcinogenesis·Hector Guillen-AhlersVictoria A Ploplis
Jan 2, 2007·The American Journal of Surgical Pathology·Maria BenevoloPatrizio Giacomini
Jan 14, 2010·Molecular Cancer Research : MCR·Ana L Romero-WeaverAna M Gamero
Nov 21, 2007·Surgical Oncology Clinics of North America·Silvia SelleriEna Wang
Sep 10, 2013·Pigment Cell & Melanoma Research·Elisa TremantePatrizio Giacomini
Dec 20, 2012·Journal of Skin Cancer·Linda GaoNelleke A Gruis
May 5, 2012·The Lancet Oncology·Elisa TremantePatrizio Giacomini
Mar 9, 2018·Cell Death and Differentiation·Najla El-HachemRobert Ballotti
Sep 25, 2009·Molecular Carcinogenesis·Jun FanRichard M Niles
Mar 25, 2016·Médecine sciences : M/S·Ingrid MasseOdile Berthier-Vergnes
Nov 2, 2019·Scientific Reports·Sherry BhallaGajendra P S Raghava

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