PMID: 9002616Dec 1, 1996Paper

cDNA cloning and characterization of a 3'/5'-O-methyltransferase for partially methylated flavonols from Chrysosplenium americanum

Plant Molecular Biology
A GauthierR K Ibrahim

Abstract

Enzymatic O-methylation of plant secondary metabolites is an important mechanism for the inactivation of reactive hydroxyl groups and for the modification of their solubility. A cDNA clone (pFOMT3') encoding the gene for the 3'/5'-O-methylation of partially methylated flavonols was isolated from Chrysosplenium americanum (Saxifragaceae). We used a PCR fragment obtained with degenerate oligonucleotides designed from conserved regions of various O-methyltransferases (OMTs). The pFOMT3' cDNA sequence shows about 67-85% similarity to other plant OMT sequences. The recombinant protein expresses strict specificity for positions 3'/5' (meta) of partially methylated flavonols, but does not accept quercetin or caffeic acid for further methylation. Southern blot analysis of the genomic DNA probed with an OMT sequence suggests the presence of a number of related genes in this species, consistent with the multiple enzymatic methylations involved in the biosynthesis of polymethylated flavonols in this plant.

References

Dec 1, 1992·Plant Molecular Biology·P CollazoJ Rigau
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Dec 1, 1994·Plant Molecular Biology·L GregersenD B Collinge
Jun 1, 1994·Plant Physiology·O PoeydomengeJ Grima-Pettenati
Jul 1, 1995·Plant Physiology

❮ Previous
Next ❯

Citations

Nov 9, 2000·Archives of Biochemistry and Biophysics·D Anzellotti, R K Ibrahim
Jul 11, 2006·Plant Molecular Biology·Huaijun Michael LiFaith C Belanger
Jan 5, 2002·Phytochemistry·Gudrun SchröderJoachim Schröder
Mar 11, 1998·Natural Product Reports·P M Dewick
Dec 7, 2007·Genome Génome / Conseil National De Recherches Canada·Kevin C LamSelvadurai Dayanandan
Oct 23, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jack KornblattRagai K Ibrahim
Dec 15, 2004·BMC Plant Biology·Dominique Anzellotti, Ragaï K Ibrahim
Jan 25, 2013·ISRN Surgery·Shafik N WassefSamuel Barnett
Aug 28, 2004·Journal of Molecular Graphics & Modelling·Heejung YangYoongho Lim
Jul 23, 1999·Archives of Biochemistry and Biophysics·J Wang, E Pichersky
Feb 7, 2020·Journal of Agricultural and Food Chemistry·Denis BarronVincenzo De Luca
Jun 1, 2019·Phytochemistry·Kevin A ReaTariq A Akhtar
Sep 28, 2006·Journal of Agricultural and Food Chemistry·Fulvio MattiviRiccardo Velasco

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.