PMID: 11606800Oct 19, 2001Paper

cDNA cloning and characterization of a high affinity aryl hydrocarbon receptor in a cetacean, the beluga, Delphinapterus leucas

Toxicological Sciences : an Official Journal of the Society of Toxicology
B A Jensen, M E Hahn

Abstract

Some cetaceans bioaccumulate substantial concentrations of planar halogenated aromatic hydrocarbons (PHAHs) in their tissues, but little is known about the effects of such burdens on cetacean health. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related PHAHs cause toxicity via activation of the aryl hydrocarbon receptor (AHR), a member of the bHLH-PAS family of transcription factors. Differences in AHR structure and function are known to contribute to species-specific differences in susceptibility to PHAH toxicity. To ascertain the potential for PHAH effects in a cetacean, we characterized an AHR from the beluga whale, Delphinapterus leucas. The 3.2 kb cDNA encodes an 845-amino acid protein with a predicted size of 95.5 kDa. Overall, the beluga AHR shares 85% amino acid sequence identity with the human AHR and 75% identity with the mouse AHR Ah(b-1) allele. Beluga AHR protein synthesized in a rabbit reticulocyte lysate system demonstrated specific, high-affinity [(3)H]TCDD binding. Saturation binding analysis was used to compare the [(3)H]TCDD binding affinity of the in vitro-expressed beluga AHR with affinities of in vitro-expressed AHRs from a dioxin-sensitive mouse strain (Ah(b-1) allele) and humans. The beluga AHR bound [...Continue Reading

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Citations

Sep 6, 2002·Chemico-biological Interactions·Mark E Hahn
Sep 12, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sonya M BilliardPeter V Hodson
Jun 17, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jeremy A LavineWade H Powell
Nov 3, 2005·Environmental Health Perspectives·Joanna Y WilsonJohn J Stegeman
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sibel I KarchnerMark E Hahn
Jan 18, 2014·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Reza FarmahinSean W Kennedy
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Sep 13, 2005·The Science of the Total Environment·Aaron T FiskDerek C G Muir
Aug 6, 2014·Chemico-biological Interactions·Reza FarmahinSean W Kennedy
Sep 11, 2007·Orvosi hetilap·Péter SimsaVilmos Fülöp
Feb 5, 2014·Environmental Science & Technology·Marie NoëlPeter S Ross
Nov 1, 2002·Marine Environmental Research·Eun-Young KimNobuyuki Miyazaki

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