PMID: 9653651Jul 8, 1998Paper

cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9)

Genomics
T K WatanabeK Tanaka

Abstract

We have employed cDNA cloning to deduce the complete primary structure of a new subunit, designated p27, of the modulator trimer complex that stimulates the association of the PA700 regulator with the catalytic 20S proteasome to form the ATP-dependent active 26S proteasome. We found two distinct cDNAs encoding two highly homologous proteins except in the C-terminal region, which are termed tentatively p27-1 and p27-2. The short p27-2 cDNA has a deletion of 65 bp near the 3'-end region of the long p27-1 cDNA, which encodes a large protein with an extended C-terminal region, designated p27-L, whereas the long p27-1 cDNA encodes a small protein named p27-S. The polypeptides of p27-L and p27-S consist of 223 and 209 amino acid residues with calculated molecular masses of 24,852 and 22,764 and isoelectric points of 6.50 and 5.28, respectively. Immunoblot analysis with anti-p27 antibody revealed that p27, together with two other ATPase components, TBP1 and p42, was associated with not only the modulator complex but also significantly with the 26S proteasome complex, suggesting that the three are common/sharing subunits in these two complexes. By the fluorescence in situ hybridization method, the p27 (PSMD9) gene was mapped to the q24...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·F Sherman
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P LichterD C Ward
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Jan 1, 1995·Molecular Biology Reports·W DubielM Rechsteiner
Oct 17, 1995·Biochimica Et Biophysica Acta·R NiK Tanaka
Sep 1, 1994·Trends in Biochemical Sciences·J M Peters
Nov 1, 1993·Journal of Structural Biology·T YoshimuraW Baumeister
Feb 9, 1996·The Journal of Biological Chemistry·G N DeMartinoC A Slaughter
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Jan 1, 1997·Advances in Immunology·K TanakaN Shimbara

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Citations

Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·B W ParkM I Greene
Feb 4, 2014·Scientifica·Aldrin V Gomes
Nov 3, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K Tanaka, T Chiba
Aug 8, 2001·The Journal of Cell Biology·B L DreesD G Drubin
May 23, 2007·Journal of Cellular Physiology·C Gragnoli, J Cronsell
Apr 30, 2013·Cell·Park F Cho-Park, Hermann Steller
Apr 30, 2010·Trends in Cell Biology·Lynn BedfordJeroen Roelofs
Feb 9, 2000·Trends in Biochemical Sciences·K FerrellC Gordon
Jul 27, 1999·The Journal of Biological Chemistry·S J RussellS A Johnston
Jul 22, 2006·Circulation Research·Aldrin V GomesPeipei Ping
Mar 10, 2010·Biochemistry. Biokhimii︠a︡·A V SorokinL P Ovchinnikov
Mar 28, 2002·Physiological Reviews·Michael H Glickman, Aaron Ciechanover
Aug 16, 2005·Molecular Endocrinology·Jamie L VolinicMelissa K Thomas
Sep 22, 1999·Molecular and Cellular Biology·J TakeuchiA Toh-e
Nov 24, 1999·Molecular and Cellular Biology·M K ThomasJ F Habener
Oct 12, 2001·Physiological Genomics·G CagneyS Fields
Mar 1, 2019·Nature·Benjamin L ParkerBrian G Drew
Aug 28, 2001·Free Radical Biology & Medicine·Q Ding, J N Keller
Jul 8, 2021·Pharmaceutical Patent Analyst·Hermann Am Mucke

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