PMID: 15354355Sep 10, 2004Paper

cDNA cloning and characterization of enolase from Chinese cabbage, Brassica campestris ssp. Pekinensis

DNA Sequence : the Journal of DNA Sequencing and Mapping
Jingya ZhaoKexuan Tang

Abstract

An enolase-encoding cDNA clone from Chinese cabbage, Brassica campestris ssp. Pekinensis, was isolated. This gene (Accession number: AY307448) had a total length of 1580bp with an open reading frame of 1335bp, and encoded a predicted polypeptide of 444 amino acids with a molecular weight of 47.38 kDa. The deduced amino acid (aa) sequence shared identity with a number of enolases ranging from Bacillus subtilis to human beings and had much higher identity with other plant enolases than with enolases from Bacillus, yeast and human beings. Comparison of its primary structure with those of other enolases revealed the presence of an insertion of 5 amino acids in enolase of Chinese cabbage. Expression of the cloned enolase gene decreased under salt stress, but increased in response to low temperature. Southern blot analysis of genomic DNA indicated that low-copies of enolase gene were present in the genome of Chinese cabbage.

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Citations

Sep 14, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Zhao-Jun WeiCheng Lu
Jun 29, 2010·Phytochemistry·Chang-Xia DuJuan Li
Feb 15, 2020·The World Allergy Organization Journal·Tania Robledo-RetanaLuis M Teran

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