PMID: 15346762Sep 7, 2004Paper

CDNA cloning and characterization of the Ve homologue gene StVe from Solanum torvum Swartz

DNA Sequence : the Journal of DNA Sequencing and Mapping
Jiong FeiKexuan Tang

Abstract

Verticillium wilt is a disastrous disease causing significant yield losses of many crops. Isolation of verticillium wilt resistance gene is a fundamental work for controlling this disease through genetic engineering. In this report, we describe the cloning and characterization of a Ve like gene (StVe) from Solanum torvum Swartz. The nucleotide sequence of StVe is 3640 bp long with an open reading frame of 3414 bp encoding a protein precursor of 1138 aa. Sharing high homologies to tomato verticillium wilt disease resistance genes Ve1 and Ve2, the leucine rich (15.89%) protein StVe has a calculated molecular weight of 126.48kDa with an isoelectric point of 5.62. It possesses a hydrophobic N-terminal signal peptide of 20 aa and 38 predicted leucine-rich repeats containing 32 potential N-glycosylation sites (28 being significant). Fifty-seven predicted phosphorylation sites (36 for S, 8 for T and 13 for Y) distribute in StVe protein. A PEST-like sequence and a mammalian endocytosis signals YCVF are found within the C-terminal region. The C terminus of StVe concludes with the residues KKF similar to the KKX motif that confers endoplasmic reticulum localization in plants as well as mammals and yeast. The sequence analysis of the StVe...Continue Reading

References

Jul 1, 1996·Trends in Biochemical Sciences·M Rechsteiner, S W Rogers
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Apr 2, 1998·Current Opinion in Immunology·B FritigM Legrand
Aug 25, 1999·Current Opinion in Microbiology·P J de Wit, M H Joosten
Aug 25, 1999·Current Opinion in Plant Biology·M Grant, J Mansfield
Dec 22, 1999·Journal of Molecular Biology·N BlomS Brunak
Feb 7, 2001·Current Opinion in Biotechnology·C M Rommens, G M Kishore
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·R A van der HoornM H Joosten
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·L M KawchukD Prufer
Jul 26, 2002·Molecular Microbiology·Rianne LudererMatthieu H A J Joosten
Jan 4, 2003·The Journal of Clinical Investigation·Nan WangAlan R Tall
Nov 1, 1990·Plant Physiology·R L BowdenT D Sharkey

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Citations

May 28, 2011·Plant Physiology·Emilie F FradinBart P H J Thomma
Jan 1, 2005·Molecular Plant Pathology·Marco KruijtPierre J G M de Wit
Dec 22, 2015·Plant Physiology and Biochemistry : PPB·Tianzi ChenBaolong Zhang
Mar 1, 2006·Molecular Plant Pathology·Emilie F Fradin, Bart P H J Thomma
Sep 21, 2012·Molecular Plant-microbe Interactions : MPMI·Zhao ZhangBart P H J Thomma
Jan 1, 2012·Biomolecules·Norio Matsushima, Hiroki Miyashita
May 9, 2009·Molecular Genetics and Genomics : MGG·Kelly Vining, Thomas Davis
Jun 15, 2014·Applied Microbiology and Biotechnology·Xiumei LuoAnping Sui
Aug 14, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Liyan WuFeihu Liu
Feb 13, 2020·International Journal of Molecular Sciences·Ranran SongXingyong Yang

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