cDNA cloning and expression analysis of the murine ribonuclease L inhibitor

Gene
A Benoit De CoignacT Salehzada

Abstract

The 2-5A/RNase L system is one of the pathways induced by interferon (IFN). It plays a major role in the antiviral and antiproliferative activities of IFNs. Recently, we have shown that the activity of the RNase L could be inhibited by a proteic inhibitor, the RNase L Inhibitor (RLI). Human RLI (Hu-RLI) was cloned and characterized. We describe here the isolation and characterization of the cDNA encoding the murine RLI (Mu-RLI). Hu-RLI and Mu-RLI protein have 98% amino acid identity. Mu-RLI is functionally homologous to Hu-RLI, and all the structural features and amino acid sequence motifs of Hu-RLI are conserved in Mu-RLI. Moreover, reticulocyte lysate translated Mu-RLI protein is also able to inhibit 2-5A binding on 2-5A-dependent RNAse-L. Northern blot analysis revealed that Mu-RLI cDNA hybridizes with one mRNA of 3.5 kb except for the testis where two mRNA of 3.5 and 2.1 kb, respectively, are detected, suggesting a tissue-specific regulation.

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Citations

Jan 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thomas H ScheuermannKevin H Gardner
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Weibo LuoGregg L Semenza
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Oct 29, 2005·Journal of Molecular Microbiology and Biotechnology·Manisha GaurRajendra Prasad
Dec 29, 2011·Frontiers in Bioscience (Scholar Edition)·Heather J Ezelle, Bret A Hassel
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Taehyun ParkRy Young
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Apr 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Min XuRy Young
Jul 26, 2005·DNA Sequence : the Journal of DNA Sequencing and Mapping·Takuji MaedaTakahiro Kusakabe
Nov 25, 2003·DNA Sequence : the Journal of DNA Sequencing and Mapping·Xi-Ling DuJin-Shui Yang

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