cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans.

The Journal of Biological Chemistry
F K Hagen, K Nehrke

Abstract

The initiation of mucin-type O-glycosylation is catalyzed by a family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGaNTase) (EC 2.4.1.41). By screening two mixed-stage Caenorhabditis elegans cDNA libraries, a total of 11 distinct sequence homologs of the ppGaNTase gene family were cloned, sequenced, and expressed as truncated recombinant proteins (gly-3, gly-4, gly-5a, gly-5b, gly-5c, gly-6a, gly-6b, gly-6c, gly-7, gly-8, and gly-9). All clones encoded type II membrane proteins that shared 60-80% amino acid sequence similarity with the catalytic domain of mammalian ppGaNTase enzymes. Two sets of cDNA clones (gly-5 and gly-6) contained variants that appeared to be produced by alternative message processing. gly-6c contained a reading frameshift and premature termination codon in the C-terminal lectin-like domain found in most other ppGaNTase proteins, and a second clone (gly-8) lacked the typical C-terminal region completely. Homogenates of nematodes and immunopurified preparations of the recombinant GLY proteins demonstrated that worms express functional ppGaNTase enzymes (GLY-3, GLY-4, GLY-5A, GLY-5B, and GLY-5C), which can O-glycosylate mammalian apomucin peptide sequences in vitro. In addition to demonstra...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·T HennetJ D Marth
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·E Martín-Blanco, A García-Bellido
Aug 1, 1996·Protein Science : a Publication of the Protein Society·B Hazes
Nov 1, 1996·Biochemical and Biophysical Research Communications·J ZaraL A Tabak

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Citations

Jun 22, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W DennisC E Warren
May 9, 2000·Biochemical and Biophysical Research Communications·M D Roos, J A Hanover
Aug 13, 2008·Glycoconjugate Journal·E Tian, Kelly G Ten Hagen
Mar 9, 2004·International Journal for Parasitology·Magdalena M Stwora-WojczykBoguslaw S Wojczyk
Mar 25, 2000·International Journal for Parasitology·R M MaizelsA Loukas
Sep 27, 2003·Molecular and Biochemical Parasitology·Boguslaw S WojczykSteven L Spitalnik
Nov 6, 2002·Biochimica Et Biophysica Acta·Prabhjit K Grewal, Jane E Hewitt
Mar 20, 1999·Biochimica Et Biophysica Acta·M LopezP Delannoy
Dec 5, 2002·Current Opinion in Structural Biology·Iain B H Wilson
Sep 3, 2010·The Journal of Biological Chemistry·Liping Zhang, Kelly G Ten Hagen
Jun 11, 2004·Annual Review of Biochemistry·Robert S Haltiwanger, John B Lowe
Oct 7, 2004·Biological & Pharmaceutical Bulletin·Naosuke NakamuraAkira Kurosaka
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·D ZhouT Hennet
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·D A BulikS B Selleck
Sep 11, 2001·Biochemical and Biophysical Research Communications·K KatoT Irimura
Jun 13, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erika Staudacher
Oct 7, 2004·Current Opinion in Structural Biology·Harry Schachter
Jul 12, 2005·Bioorganic & Medicinal Chemistry·Howard C Hang, Carolyn R Bertozzi
Sep 26, 2007·International Journal for Parasitology·Andrea MedeirosEduardo Osinaga
Sep 15, 2004·The Journal of Biological Chemistry·Katharina PaschingerIain B H Wilson
Nov 6, 2002·FEBS Letters·Lamei ChengHisashi Narimatsu
Nov 14, 2006·The Journal of Biological Chemistry·E Tian, Kelly G Ten Hagen

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