PMID: 2113405May 24, 1990Paper

cDNA cloning and expression of a Talaromyces emersonii beta-glucosidase determinant in Escherichia coli

Biochimica Et Biophysica Acta
J MorrisonA P McHale

Abstract

Intact mRNA has been isolated from the thermophilic fungus Talaromyces emersonii following growth on lactose-containing media and this has been used as template to produce cDNA. This cDNA has been cloned into the Escherichia coli expression system, pUC18 and this DNA was used to transform E. coli. A 2.1 kb fragment was isolated and shown to encode functional beta-glucosidase activity in E. coli. When the fragment was sub-cloned into a leaky strain of E. coli K-12, beta-glucosidase activity was detected in culture supernatants. The fragment was characterised further using restriction enzyme analysis and was also used as a probe in Northern and Southern blotting analyses of T. emersonii mRNA and genomic DNA, respectively. Results obtained from these analyses verified that the cloned insert DNA was of T. emersonii origin.

References

Jul 1, 1954·Canadian Journal of Biochemistry and Physiology·W L BALLK KAY

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Citations

Jul 29, 2010·Environmental Technology·Desire BarnardDonald Cowan
Oct 19, 2016·Biotechnology and Applied Biochemistry·James Sy-Keen WoonFarah Diba Abu Bakar

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