cDNA cloning and expression patterns of a peroxiredoxin, a catalase and a glutathione peroxidase from Haemonchus contortus

Parasitology Research
N H Bagnall, A C Kotze

Abstract

The range of antioxidant enzyme systems available to Haemonchus contortus for detoxification of hydrogen peroxide was investigated using cDNA cloning of candidate genes. PCR with primers based on conserved amino acid regions and spliced leader sequences was used to obtain full-length sequences for a 2-Cys peroxiredoxin, a catalase, and a selenium-independent glutathione peroxidase, indicating that H. contortus expresses a number of antioxidant systems with the potential to detoxify peroxide (nucleotide sequence data reported in this paper are available in the GenBank, EMBL and DDBJ databases under the accession numbers AY603335, AY603336 and AY603337). Quantitative PCR analysis comparing L3-stage larvae with adult worms showed significantly elevated peroxiredoxin levels in adults, equivalent catalase levels in the two stages, and significantly less glutathione peroxidase in adults, suggesting a significant role for peroxiredoxin in allowing the nematode to detoxify hydrogen peroxide encountered in the parasitic environment. Exposure of L4-stage worms to hydrogen peroxide in vitro (generated using glucose/glucose oxidase) caused no change in mRNA levels for each of the genes, though the exposed worms showed up to eightfold highe...Continue Reading

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Citations

Dec 1, 2012·Journal of Chemical Information and Modeling·Minsup KimArt E Cho
Jul 8, 2010·Veterinary Research Communications·Marta Lizandra do Reo LealJoão Batista Teixeira da Rocha
Jun 12, 2009·Drug Metabolism Reviews·Viktor CvilinkLenka Skálová
May 18, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jungkon KimKyungho Choi
Jan 31, 2014·Bulletin of Environmental Contamination and Toxicology·Woo-Keun KimJinho Jung
Oct 29, 2005·Molecular and Biochemical Parasitology·Andrew C Kotze, Neil H Bagnall
Nov 16, 2010·Molecular and Biochemical Parasitology·Amanda L HudsonMary W Davey
Jun 21, 2014·Experimental Parasitology·Marta Lizandra do Rêgo LealJoão Batista Teixeira da Rocha
Dec 15, 2010·International Journal for Parasitology·Géraldine DubreuilMarie-Noëlle Rosso
Jun 9, 2006·International Journal for Parasitology·S Nikolaou, R B Gasser

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