cDNA cloning and functional analysis of ascidian sperm proacrosin

The Journal of Biological Chemistry
E KodamaH Sawada

Abstract

cDNA cloning and functional analysis of proacrosin from the ascidian Halocynthia roretzi were undertaken. The isolated cDNA of the ascidian preproacrosin consists of 2367 nucleotides, and an open reading frame encodes 505 amino acids, which corresponds to the molecular mass of 55,003 Da. The mRNA of proacrosin was found to be specifically expressed in the gonad by Northern blotting and in the spermatocytes or spermatids by in situ hybridization. The amino acid sequences around His(76), Asp(132), and Ser(227), which make up a catalytic triad, showed high homology to those of the trypsin family. Ascidian acrosin has paired basic residues (Lys(56)-His(57)) in the N-terminal region, which is one of the most characteristic features of mammalian acrosin. This region seems to play a key role in the binding of (pro)acrosin to the vitelline coat, because the peptide containing the paired basic residues, but not the peptide substituted with Ala, was capable of binding to the vitelline coat. Unlike mammalian proacrosin, ascidian proacrosin contains two CUB domains in the C-terminal region, in which CUB domain 1 seems to be involved in its binding to the vitelline coat. Four components of the vitelline coat that are capable of binding to C...Continue Reading

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Citations

Mar 10, 2004·The International Journal of Biochemistry & Cell Biology·Naoyuki SakaiHitoshi Sawada
Jun 17, 2008·Development, Growth & Differentiation·Noritaka HirohashiMotonori Hoshi
May 16, 2002·Zoological Science·Hitoshi Sawada
Jun 7, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Tomohiro SasanamiHideo Kubo
Nov 18, 2006·Current Opinion in Immunology·Konstantin Khalturin, Thomas C G Bosch
Mar 30, 2007·Molecular Reproduction and Development·Yoshito Harada, Hitoshi Sawada
Jan 26, 2013·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Mari AkasakaHitoshi Sawada
Sep 11, 2004·Biochemical and Biophysical Research Communications·Naisong LinXuetao Cao
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
Jun 5, 2003·FEBS Letters·Josephine M HermansRoy Jones

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