cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome

Gene
A SaitoK Tanaka

Abstract

We have employed cDNA cloning to deduce the complete primary structures of p44.5 and p55, two subunits of PA700, a 700-kDa multisubunit regulatory complex of the human 26S proteasome. These polypeptides consist of 422 and 456 amino acids with calculated molecular masses of 47463 and 52903, and isoelectric points of 6.06 and 7.56, respectively. Computer-assisted homology analysis revealed high sequence similarities of p44.5 and p55 with yeast proteins whose functions are yet unknown. Disruption of the yeast genes, termed NAS4 and NAS5 (non-ATPase subunits 4 and 5), resulted in lethality, indicating that each of the two subunits is essential for proliferation of yeast cells.

References

Jan 1, 1991·Methods in Enzymology·F Sherman
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Oct 6, 1995·The Journal of Biological Chemistry·Q DeverauxM Rechsteiner
Jan 1, 1995·Molecular Biology Reports·W DubielM Rechsteiner
Jul 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Confalonieri, M Duguet
May 16, 1995·Biochemical and Biophysical Research Communications·C TsurumiK Tanaka
Sep 1, 1994·Trends in Biochemical Sciences·J M Peters
Nov 1, 1993·Journal of Structural Biology·T YoshimuraW Baumeister
Dec 15, 1995·The Journal of Biological Chemistry·Q DeverauxM Rechsteiner
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·S van NockerR D Vierstra
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Mar 10, 1997·FEBS Letters·L Hoffman, M Rechsteiner
Mar 1, 1997·Molecular Biology Reports·K Tanaka, C Tsurumi

❮ Previous
Next ❯

Citations

Aug 28, 2001·Free Radical Biology & Medicine·Q Ding, J N Keller
Dec 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ganesh Ramnath PathareAndreas Bracher
Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nicholas MitsiadesKenneth C Anderson
Oct 2, 2012·Biochemical and Biophysical Research Communications·Bin ZhaoKristina Lindsten
Aug 21, 2002·BMC Genetics·Susan WeeDieter A Wolf
Dec 18, 2002·The Journal of Biological Chemistry·Patricia G SantamariaMiguel Remacha
Jun 5, 2003·The Journal of Biological Chemistry·Hsueh-Chi S YenEric C Chang
Jun 20, 1998·Molecular and Cellular Biology·M H GlickmanD Finley
Feb 2, 2021·BMC Developmental Biology·Linlin ZhaoTonggang Qi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.