PMID: 16519316Mar 8, 2006Paper

cDNA cloning and gene expression of ribosomal S9 protein gene in the mollusk Corbicula fluminea: a new potential biomarker of metal contamination up-regulated by cadmium and repressed by zinc

Environmental Toxicology and Chemistry
Maud Achard-JorisJean-Paul Bourdineaud

Abstract

Biological indicators can be used to assess polluted sites, but their success depends on their specificity. The aim of the present study was to identify a specific cadmium biomarker of the freshwater bivalve Corbicula fluminea. Differential display technique was used to identify new molecular indicators for cadmium exposure. Gene expression of C. fluminea transplanted into a cadmium- and zinc-contaminated river was compared to gene expression of bivalves from a reference site. One differentially expressed band was identified, and the entire cDNA sequence coding for the ribosomal protein S9 (rpS9) was cloned. The rpS9 gene expression was studied by real-time reverse transcription-polymerase chain reaction. After controlled cadmium (15 microg/L) and/or zinc (1 mg/L) laboratory contaminations of the bivalves, this gene was shown to be up-regulated by cadmium and down-regulated by zinc. A 48-d transplantation of animals into a polymetallic field contamination in the Lot river basin (Midi-Pyrénées region, France) showed that rpS9 gene regulation was correlated with the fluctuating cadmium and zinc water contents. To our knowledge, the existence of a gene that is up-regulated by cadmium and repressed by zinc is demonstrated here for ...Continue Reading

Citations

May 3, 2007·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Jeong-Ha YooSung-Cheol Koh
May 23, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Kiyun Park, Inn-Sil Kwak
May 18, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J L Martínez-GuitarteG Morcillo
Aug 29, 2006·Biochimie·J-P BourdineaudJ-L Moreau
Jun 21, 2020·Biological Trace Element Research·Lefei JiaoQicun Zhou
May 19, 2020·The Science of the Total Environment·Clément PeretoMagalie Baudrimont

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