cDNA cloning and tissue expression analyses of the encoding regions for three novel porcine genes- MJD1, CDC42 and NECD

Animal Biotechnology
G Y LiuX Zhang

Abstract

The cDNAs for Machado-Joseph disease protein 1 homolog (MJD1), cell division control protein 42 homolog precursor(CDC42) and necdin (NECD) genes of pig were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the MJD1, CDC42, and NECD genes from mouse and other mammals and the referenced porcine EST sequence information. Tissue expression analysis showed the swine MJD1, CDC42, and NECD genes were obviously differentially expressed in different tissues including muscle, heart, liver, backfat, kidney, lung, small intestine, and large intestine. Our experiment established the primary foundation for further research on these three swine genes.

References

Mar 21, 2002·Acta Neurologica Scandinavica·K IkedaM Nakagawa
Nov 16, 2002·Archives of Neurology·Atsushi IshikawaShoji Tsuji
Jun 11, 2003·BMC Gastroenterology·Yataro DaigoMasayuki A Fujino
Jul 22, 2004·European Journal of Biochemistry·Mario AlbrechtThomas Lengauer
May 26, 2006·European Journal of Human Genetics : EJHG·Sandra MartinsAntónio Amorim
Jan 4, 2007·Journal of Cell Science·Ariadna Mendoza-NaranjoRicardo B Maccioni

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Datasets Mentioned

BETA
BP434946
DQ917623
DB794900
EX654638

Methods Mentioned

BETA
GTPase
PCR

Software Mentioned

BLAST

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