PMID: 9434148Jan 20, 1998Paper

cDNA cloning and tissue-specific expression of the gene encoding human adenylate kinase isozyme 2

Biochimica Et Biophysica Acta
T NomaA Nakazawa

Abstract

We isolated two kinds of cDNAs encoding human adenylate kinase (AK) isozyme 2 from a HeLa cell cDNA library using bovine AK2 cDNA as a probe. Nucleotide sequencing revealed that the cDNAs encoded 239- and 232-amino acid proteins with deduced molecular mass of 26.5 (AK2A) and 25.6kDa (AK2B), respectively. Northern blot analysis demonstrated that AK2 mRNA is strongly expressed in liver, heart, skeletal muscle and pancreas, and moderately in kidney, placenta and brain, and weakly in lung. However, Western blot analysis showed that AK2 protein was present in large amounts in liver, heart, kidney, and in a small amount in lung, and undetectable in brain and skeletal muscle. These results suggested the presence of the tissue-specific gene-expression including post-transcriptional regulation in expression of the AK2 gene.

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Citations

Oct 10, 2008·Journal of Molecular Neuroscience : MN·Alireza MinagarMerilyn H Jennings
Jan 17, 2012·Journal of Molecular Neuroscience : MN·Xi PengXuefeng Wang
Sep 29, 2000·Pharmacology & Therapeutics·A R Van RompayA Karlsson
Aug 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·K F LauP C Shaw
Sep 30, 2010·The Journal of Biological Chemistry·Alison BurkartSilvia Corvera
Sep 17, 2005·American Journal of Respiratory Cell and Molecular Biology·Esther BarreiroSabah N A Hussain
Aug 19, 2005·BMC Physiology·Colleen A McMullenFrancisco H Andrade
Feb 6, 2014·The International Journal of Biochemistry & Cell Biology·Christakis PanayiotouAnna Karlsson
Sep 29, 2009·Current Opinion in Immunology·Kaan Boztug, Christoph Klein
May 7, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Koichi FujisawaTakafumi Noma
Sep 22, 2009·The International Journal of Biochemistry & Cell Biology·Christakis PanayiotouAnna Karlsson
Apr 8, 2014·The Journal of Medical Investigation : JMI·Taigo HoriguchiTakafumi Noma
Sep 29, 2018·International Journal of Molecular Sciences·Marju PuurandTuuli Kaambre
Apr 30, 2003·Journal of Applied Physiology·Francisco H AndradeJohn D Porter
Nov 15, 2007·The Journal of Biological Chemistry·Yunjian XuAnna Karlsson
Feb 10, 2021·Blood Advances·Nabih MaslahGuillaume P Andrieu

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