cDNA cloning, genomic organization, and chromosomal localization of the mouse LIM motif-containing kinase gene, Limk2

Biochemical and Biophysical Research Communications
U KoshimizuT Nakamura

Abstract

LIM-kinases, including LIMK1 and LIMK2, are unique LIM-family proteins with two tandem LIM motifs at the N-terminal and a serine/threonine kinase domain at the C-terminal. In this study, we cloned two types of mouse Limk2 cDNA; one is an intact form (Limk2a) and the other has only one complete LIM domain (Limk2b). Northern blot analysis showed Limk2a mRNA was ubiquitously present in various adult tissues, while Limbk2b was predominantly expressed in brain. We also identified genomic organization of the Limk2 gene; it is similar to that of the related gene Limk1. The transcription unit contains 16 exons plus two alternative exons, thus, variation in the initiator exon usage gives rise to alternative transcripts of Limk2. Fluorescent in situ hybridization analysis showed the Limk2 gene were mapped to mouse chromosome 1D. These findings provide some important clues to the in vivo functions of LIM kinases.

Citations

Feb 16, 2007·Gene Expression Patterns : GEP·Elisabeth B OttChristoph P Bagowski
Jan 11, 2001·Developmental Biology·T TakahashiT Nakamura
Jan 29, 2002·Biochemical and Biophysical Research Communications·Tomoyuki SumiToshikazu Nakamura
Feb 4, 2006·Experimental Cell Research·Tomoyuki SumiToshikazu Nakamura
May 4, 1999·The International Journal of Biochemistry & Cell Biology·C A Stanyon, O Bernard
Jan 10, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Karla AcevedoOra Bernard
Feb 13, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Rebecca W Scott, Michael F Olson
May 8, 2001·The Journal of Biological Chemistry·T SumiT Nakamura
Nov 25, 2003·Biology of Reproduction·J A GuttmanA W Vogl
Aug 26, 1998·Biochemical and Biophysical Research Communications·H TakahashiT Nakamura
Jan 11, 2002·Developmental Biology·Hisaaki TakahashiToshikazu Nakamura
Jun 4, 1998·Biochemical and Biophysical Research Communications·C IkebeK Mizuno

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