PMID: 2123099Nov 1, 1990Paper

cDNA cloning of a 30 kDa erythrocyte membrane protein associated with Rh (Rhesus)-blood-group-antigen expression

The Biochemical Journal
N D AventD J Anstee

Abstract

The Rh-blood-group antigens (often described as Rhesus antigens) are associated with erythrocyte membrane proteins of approx. 30 kDa. We have determined the N-terminal 54 amino acid residues of the 30 kDa Rh D polypeptide (D30 polypeptide). We used primers based on these sequence data and the polymerase chain reaction (PCR) on human reticulocyte cDNA and genomic DNA to clone two types of PCR product of identical size. The two PCR products had related translated amino acid sequences between the 3' ends of the primers, one of which was identical with that found for the D30 polypeptide. We designate the two related mRNA species which gave rise to the PCR products as Rh30A and Rh30B, the latter corresponding to the D30 polypeptide. We have isolated cDNA clones for the Rh30A protein which encode a hydrophobic membrane protein of 417 amino acids. The Rh30A protein has the same N-terminal 41 amino acids as the D30 polypeptide, but beyond this point the sequence differs, but is clearly related. The Rh30A protein probably corresponds to the R6A32 polypeptide, another member of the Rh 30 kDa family of proteins, which may carry the C/c and/or E/e antigens. Hydropathy analysis suggests that the Rh30A protein has up to 12 transmembrane doma...Continue Reading

Citations

Sep 1, 1993·Nature Genetics·I MouroC Le Van Kim
Mar 17, 1994·The New England Journal of Medicine·S SimsekA E von dem Borne
Aug 26, 1993·The New England Journal of Medicine·P R BennettJ P Cartron
Sep 21, 2001·Journal of Pediatric Hematology/oncology·N D Avent
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Jan 1, 1994·Vox Sanguinis·Y ColinJ P Cartron
Jun 1, 1991·Transfusion Medicine·N C Hughes-Jones
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Feb 14, 2016·Genome Biology and Evolution·Takashi KitanoNaruya Saitou
Oct 21, 2017·Therapeutic Advances in Hematology·Gregory A DenommeWilly A Flegel
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